Protein Info for ECD_01961 in Escherichia coli BL21

Annotation: putative glycosyl transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 248 signal peptide" amino acids 1 to 16 (16 residues), see Phobius details TIGR04009: colanic acid biosynthesis glycosyltransferase WcaE" amino acids 1 to 248 (248 residues), 515.9 bits, see alignment E=7e-160 PF00535: Glycos_transf_2" amino acids 4 to 99 (96 residues), 52 bits, see alignment E=4.3e-18

Best Hits

Swiss-Prot: 99% identical to WCAE_ECOLI: Putative colanic acid biosynthesis glycosyl transferase WcaE (wcaE) from Escherichia coli (strain K12)

KEGG orthology group: K13683, putative colanic acid biosynthesis glycosyltransferase [EC: 2.4.-.-] (inferred from 99% identity to eco:b2055)

MetaCyc: 99% identical to GDP-Fuc:2/3-O-Ac-alpha-L-Fuc-(1->3)-alpha-D-Glc-PP-Und alpha-(1,4)-fucosyltransferase (Escherichia coli K-12 substr. MG1655)
2.4.1.-

Predicted SEED Role

"Colanic acid biosynthesis glycosyl transferase WcaE" in subsystem Colanic acid biosynthesis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.-.-

Use Curated BLAST to search for 2.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (248 amino acids)

>ECD_01961 putative glycosyl transferase (Escherichia coli BL21)
MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSNDGTREYLENLNGIFNL
RFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANFVRKLKMQKDNMMITGDAL
LDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKWRYDLEYKVSSDYALAAKMYK
AGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQILHVPGFWAELSWHLRQRTTSK
TKALYNKS