Protein Info for ECD_01896 in Escherichia coli BL21

Annotation: putative multdrug exporter, MATE family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 547 transmembrane" amino acids 54 to 74 (21 residues), see Phobius details amino acids 95 to 120 (26 residues), see Phobius details amino acids 138 to 159 (22 residues), see Phobius details amino acids 172 to 192 (21 residues), see Phobius details amino acids 213 to 235 (23 residues), see Phobius details amino acids 246 to 268 (23 residues), see Phobius details amino acids 284 to 304 (21 residues), see Phobius details amino acids 317 to 338 (22 residues), see Phobius details amino acids 350 to 373 (24 residues), see Phobius details amino acids 404 to 429 (26 residues), see Phobius details amino acids 439 to 460 (22 residues), see Phobius details amino acids 472 to 495 (24 residues), see Phobius details amino acids 498 to 520 (23 residues), see Phobius details TIGR00797: MATE efflux family protein" amino acids 100 to 504 (405 residues), 233.2 bits, see alignment E=2.5e-73 PF01554: MatE" amino acids 100 to 260 (161 residues), 126.7 bits, see alignment E=3.6e-41 amino acids 330 to 491 (162 residues), 72.9 bits, see alignment E=1.2e-24

Best Hits

Swiss-Prot: 100% identical to YEEO_ECOLI: Probable FMN/FAD exporter YeeO (yeeO) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to ecd:ECDH10B_2129)

MetaCyc: 100% identical to FMN/FAD exporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-272; TRANS-RXN0-595

Predicted SEED Role

"FIG01069516: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (547 amino acids)

>ECD_01896 putative multdrug exporter, MATE family (Escherichia coli BL21)
MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSFADSPSLFEMWFHFLQL
RSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIFMENACVLLMGVLSTFLVS
WLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLGKRDRRRARVATRQSLVIMTL
FAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLELTVLSYPAAAITLIGSGALRGAG
NTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFVGAGLGLTISRYIGAVAILWVLAIG
FNPALRISLKSYFKPLNFSIIWEVMGIGIPASVESVLFTSGRLLTQMFVAGMGTSVIAGN
FIAFSIAALINLPGSALGSASTIITGRRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTA
PFAGVMASFYTQDPQVKHVVVILIWLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLS
MWGCRVVVGYVLGIMLGWGVVGVWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCE
KKPVVSE