Protein Info for ECD_01816 in Escherichia coli BL21

Annotation: protease II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 686 PF02897: Peptidase_S9_N" amino acids 3 to 403 (401 residues), 418.7 bits, see alignment E=2.4e-129 PF00326: Peptidase_S9" amino acids 463 to 676 (214 residues), 233.9 bits, see alignment E=1.5e-73

Best Hits

Swiss-Prot: 99% identical to PTRB_ECOLI: Protease 2 (ptrB) from Escherichia coli (strain K12)

KEGG orthology group: K01354, oligopeptidase B [EC: 3.4.21.83] (inferred from 99% identity to eoh:ECO103_2035)

MetaCyc: 99% identical to oligopeptidase B (Escherichia coli K-12 substr. MG1655)
Oligopeptidase B. [EC: 3.4.21.83]

Predicted SEED Role

"Protease II (EC 3.4.21.83)" (EC 3.4.21.83)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.21.83

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (686 amino acids)

>ECD_01816 protease II (Escherichia coli BL21)
MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQENSYGHRVMASQQALQDR
ILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQSAFSEEWDEWETLLDANER
AAHSEFYSMGGMAITPNNTIMALAEDFLSRRQYGIRFRNLETGNWYPELLDNVEPSFVWA
NDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYEEKDDTYYVSLHKTTSKHYVVIH
LASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLDHYQHRFYLRSNRHGKNFGLYRTRM
RDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQRGLTSLRQINRKTREVIGIAFDDPAY
VTWIAYNPEPETARLRYGYSSMTTPDTLFELDMDTGERRVLKQTEVPGFDAANYRSEHLW
IVARDGVEVPVSLVYHRKHFRKGHNPLLVYGYGSYGASIDADFSFSRLSLLDRGFVYAIV
HVRGGGELGQQWYEDGKFLKKKNTFNDYLDACDALLKLGYGSPSLCYAMGGSAGGMLMGV
AINQRPELFHGVIAQVPFVDVVTTMLDESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDN
VTAQAYPHLLVTTGLHDSQVQYWEPAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFK
SYEGVAMEYAFLVALAQGTLPATPAD