Protein Info for ECD_01736 in Escherichia coli BL21
Annotation: cytoplasmic L-asparaginase 1
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to ASPG1_ECO57: L-asparaginase 1 (ansA) from Escherichia coli O157:H7
KEGG orthology group: K01424, L-asparaginase [EC: 3.5.1.1] (inferred from 100% identity to eco:b1767)MetaCyc: 100% identical to L-asparaginase 1 (Escherichia coli K-12 substr. MG1655)
Asparaginase. [EC: 3.5.1.1, 3.5.1.38, 3.5.5.4]
Predicted SEED Role
No annotation
MetaCyc Pathways
- superpathway of L-aspartate and L-asparagine biosynthesis (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- L-asparagine degradation I (1/1 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-citrulline biosynthesis (6/8 steps found)
- L-glutamate and L-glutamine biosynthesis (5/7 steps found)
- L-asparagine degradation III (mammalian) (2/3 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
- N-(1-deoxy-D-fructos-1-yl)-L-asparagine degradation (2/5 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (1/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (1/4 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Cyanoamino acid metabolism
- D-Glutamine and D-glutamate metabolism
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 3.5.1.1
Use Curated BLAST to search for 3.5.1.1 or 3.5.1.38 or 3.5.5.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (338 amino acids)
>ECD_01736 cytoplasmic L-asparaginase 1 (Escherichia coli BL21) MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRPEMPDFTIHEYTPLMDS SDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIP LAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNRTTKAHADGFDAFASPNLPPL LEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIYPGISADVVRNFLRQPVKALILR SYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSGKVNMGGYATGNALAHAGVIGGADM TVEATLTKLHYLLSQELDTETIRKAMSQNLRGELTPDD