Protein Info for ECD_01735 in Escherichia coli BL21

Annotation: protease IV (signal peptide peptidase)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 618 transmembrane" amino acids 21 to 43 (23 residues), see Phobius details TIGR00705: signal peptide peptidase SppA, 67K type" amino acids 16 to 609 (594 residues), 950.9 bits, see alignment E=2.4e-290 PF01343: Peptidase_S49" amino acids 141 to 295 (155 residues), 156.9 bits, see alignment E=4.1e-50 amino acids 392 to 543 (152 residues), 182.3 bits, see alignment E=6e-58 TIGR00706: signal peptide peptidase SppA, 36K type" amino acids 335 to 541 (207 residues), 176.5 bits, see alignment E=5.7e-56

Best Hits

Swiss-Prot: 100% identical to SPPA_ECOLI: Protease 4 (sppA) from Escherichia coli (strain K12)

KEGG orthology group: K04773, protease IV [EC: 3.4.21.-] (inferred from 100% identity to eco:b1766)

MetaCyc: 100% identical to protease IV, a signal peptide peptidase (Escherichia coli K-12 substr. MG1655)
3.4.21.-

Predicted SEED Role

"Signal peptide peptidase SppA (EC 3.4.21.-)" (EC 3.4.21.-)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (618 amino acids)

>ECD_01735 protease IV (signal peptide peptidase) (Escherichia coli BL21)
MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQVSGGDSKETASRGALL
LDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVNTIRQAKDDRNITGIVMDL
KNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQYYLASFANKIWLSPQGVVDLH
GFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRDDMSPAAREADSRWIGELWQNYL
NTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYALENKLVDALASSAEIEKALTKEFG
WSKTDKNYRAISYYDYALKTPADTGDSIGVVFANGAIMDGEETQGNVGGDTTAAQIRDAR
LDPKVKAIVLRVNSPGGSVTASEVIRAELAAARAAGKPVVVSMGGMAASGGYWISTPANY
IVANPSTLTGSIGIFGVITTVENSLDSIGVHTDGVSTSPLADVSITRALPPEAQLMMQLS
IENGYKRFITLVADARHSTPEQIDKIAQGHVWTGQDAKANGLVDSLGDFDDAVAKAAELA
KVKQWHLEYYVDEPTFFDKVMDNMSGSVRAMLPDAFQAMLPAPLASVASTVKSESDKLAA
FNDPQNRYAFCLTCANMR