Protein Info for ECD_01390 in Escherichia coli BL21

Annotation: IS609 transposase B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 382 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details PF12323: HTH_OrfB_IS605" amino acids 1 to 28 (28 residues), 28.1 bits, see alignment (E = 1.7e-10) PF01385: OrfB_IS605" amino acids 142 to 257 (116 residues), 120.3 bits, see alignment E=8.5e-39 TIGR01766: transposase, IS605 OrfB family" amino acids 224 to 311 (88 residues), 39.2 bits, see alignment E=4e-14 PF07282: OrfB_Zn_ribbon" amino acids 284 to 352 (69 residues), 86.3 bits, see alignment E=1.6e-28

Best Hits

Swiss-Prot: 100% identical to INSQ_ECOLI: Putative transposase InsQ for insertion sequence element IS609 (insQ) from Escherichia coli (strain K12)

KEGG orthology group: K07496, putative transposase (inferred from 100% identity to eco:b1432)

Predicted SEED Role

"putative virulence protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (382 amino acids)

>ECD_01390 IS609 transposase B (Escherichia coli BL21)
MRRFAGACRFVFNRALARQNENHEAGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQ
QSLKDLERAYKNFFRKRAAFPRFKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNS
RQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEP
VNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKN
HAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGG
QVLAVPPAYTSQRCAYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLAC
GEMVQSGRPLKQEPTEMIQATA