Protein Info for ECD_01125 in Escherichia coli BL21

Annotation: peptidase T

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 408 TIGR01882: peptidase T" amino acids 2 to 406 (405 residues), 523.3 bits, see alignment E=2.2e-161 PF01546: Peptidase_M20" amino acids 139 to 402 (264 residues), 62.4 bits, see alignment E=9e-21 PF07687: M20_dimer" amino acids 206 to 305 (100 residues), 57.6 bits, see alignment E=1.7e-19

Best Hits

Swiss-Prot: 100% identical to PEPT_ECOLC: Peptidase T (pepT) from Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)

KEGG orthology group: K01258, tripeptide aminopeptidase [EC: 3.4.11.4] (inferred from 100% identity to eco:b1127)

MetaCyc: 100% identical to peptidase T (Escherichia coli K-12 substr. MG1655)
Tripeptide aminopeptidase. [EC: 3.4.11.4]

Predicted SEED Role

"Tripeptide aminopeptidase (EC 3.4.11.4)" (EC 3.4.11.4)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.11.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (408 amino acids)

>ECD_01125 peptidase T (Escherichia coli BL21)
MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEEMGLINVTLSEKGTLMA
TLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGDIALGIGDEVLSPVMFPVL
HQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNIPHGDIRVAFTPDEEVGKGAK
HFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIVGNNVHPGTAKGVMVNALSLAAR
IHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMHYIIRDFDRKQFEARKRKMMEIAKK
VGKGLHPDCYIELVIEDSYYNMREKVVEHPHILDIAQQAMRDCDIEPELKPIRGGTDGAQ
LSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEKAVQVIVRIAELTAQRK