Protein Info for ECD_01123 in Escherichia coli BL21

Annotation: spermidine/putrescine ABC transporter permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 275 signal peptide" amino acids 5 to 6 (2 residues), see Phobius details transmembrane" amino acids 7 to 26 (20 residues), see Phobius details amino acids 55 to 80 (26 residues), see Phobius details amino acids 90 to 114 (25 residues), see Phobius details amino acids 134 to 160 (27 residues), see Phobius details amino acids 187 to 214 (28 residues), see Phobius details amino acids 242 to 262 (21 residues), see Phobius details PF00528: BPD_transp_1" amino acids 73 to 272 (200 residues), 54.1 bits, see alignment E=8.6e-19

Best Hits

Swiss-Prot: 100% identical to POTB_SHIFL: Spermidine/putrescine transport system permease protein PotB (potB) from Shigella flexneri

KEGG orthology group: K11071, spermidine/putrescine transport system permease protein (inferred from 100% identity to eco:b1125)

MetaCyc: 100% identical to spermidine preferential ABC transporter membrane subunit PotB (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]; 7.6.2.11 [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1)" in subsystem Polyamine Metabolism (TC 3.A.1.11.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (275 amino acids)

>ECD_01123 spermidine/putrescine ABC transporter permease (Escherichia coli BL21)
MIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFVKMVFTLDNYTRLLDPLYFEVLLHSLN
MALIATLACLVLGYPFAWFLAKLPHKVRPLLLFLLIVPFWTNSLIRIYGLKIFLSTKGYL
NEFLLWLGVIDTPIRIMFTPSAVIIGLVYILLPFMVMPLYSSIEKLDKPLLEAARDLGAS
KLQTFIRIIIPLTMPGIIAGCLLVMLPAMGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDW
PFGAATSITLTIVMGLMLLVYWRASRLLNKKVELE