Protein Info for ECD_00743 in Escherichia coli BL21
Annotation: 8-amino-7-oxononanoate synthase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to BIOF_ECOHS: 8-amino-7-oxononanoate synthase (bioF) from Escherichia coli O9:H4 (strain HS)
KEGG orthology group: K00652, 8-amino-7-oxononanoate synthase [EC: 2.3.1.47] (inferred from 100% identity to ecx:EcHS_A0830)MetaCyc: 98% identical to 8-amino-7-oxononanoate synthase (Escherichia coli K-12 substr. MG1655)
8-amino-7-oxononanoate synthase. [EC: 2.3.1.47]; 2.3.1.47 [EC: 2.3.1.47]
Predicted SEED Role
"8-amino-7-oxononanoate synthase (EC 2.3.1.47)" in subsystem Biotin biosynthesis (EC 2.3.1.47)
MetaCyc Pathways
- biotin biosynthesis I (15/15 steps found)
- 8-amino-7-oxononanoate biosynthesis I (11/11 steps found)
- 8-amino-7-oxononanoate biosynthesis IV (4/5 steps found)
- biotin biosynthesis II (4/6 steps found)
- 8-amino-7-oxononanoate biosynthesis II (1/2 steps found)
- 8-amino-7-oxononanoate biosynthesis III (1/2 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.3.1.47
Use Curated BLAST to search for 2.3.1.47
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (384 amino acids)
>ECD_00743 8-amino-7-oxononanoate synthase (Escherichia coli BL21) MSWQDKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNFSSNDYLGLSHHPQIIR AWKQSAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRALLFISGFAANQAVIAAMM AKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLARLLASPCPGQQMVVTEGVFSM DGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRGSCWLQKVKPELLVVTFGKGFGV SGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQALRASLAVIRSDEGDARREKLAALIT RFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQLAEKLRQQGCWVTAIRPPTVPAGTARL RLTLTAAHEMQDIDRLLEVLHGNG