Protein Info for ECD_00243 in Escherichia coli BL21
Annotation: PaoABC aldehyde oxidoreductase, Moco-containing subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 99% identical to PAOC_ECO57: Aldehyde oxidoreductase molybdenum-binding subunit PaoC (paoC) from Escherichia coli O157:H7
KEGG orthology group: K11177, xanthine dehydrogenase YagR molybdenum-binding subunit [EC: 1.17.1.4] (inferred from 100% identity to ebd:ECBD_3373)MetaCyc: 99% identical to aldehyde dehydrogenase, molybdenum cofactor-binding subunit (Escherichia coli K-12 substr. MG1655)
Carboxylate reductase. [EC: 1.2.99.6]; 1.2.98.- [EC: 1.2.99.6]; 1.2.98.- [EC: 1.2.99.6]
Predicted SEED Role
"Periplasmic aromatic aldehyde oxidoreductase, molybdenum binding subunit YagR"
MetaCyc Pathways
- purine nucleotides degradation II (aerobic) (11/11 steps found)
- adenosine nucleotides degradation II (5/5 steps found)
- purine nucleotides degradation I (plants) (10/12 steps found)
- adenosine nucleotides degradation I (7/8 steps found)
- guanosine nucleotides degradation II (4/4 steps found)
- guanosine nucleotides degradation III (4/4 steps found)
- inosine 5'-phosphate degradation (4/4 steps found)
- superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
- guanosine nucleotides degradation I (3/4 steps found)
- superpathway of purines degradation in plants (13/18 steps found)
- ureide biosynthesis (5/7 steps found)
- purine nucleobases degradation II (anaerobic) (17/24 steps found)
- caffeine degradation III (bacteria, via demethylation) (1/7 steps found)
- theophylline degradation (1/9 steps found)
- caffeine degradation IV (bacteria, via demethylation and oxidation) (1/10 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.17.1.4
Use Curated BLAST to search for 1.17.1.4 or 1.2.99.6
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (732 amino acids)
>ECD_00243 PaoABC aldehyde oxidoreductase, Moco-containing subunit (Escherichia coli BL21) MKFDKPAGENPIDQLKVVGRPYDRIDGPLKTTGTARYAYEWHEESPNAAYGYIVGSAMAK GRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGGPTIEHYHQAIALVVAETF EQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPEDTPDKNVGDFDGAFTSAAVKIDATY TTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRTDLAKTLKVPVENVRIISPYIGG GFGGKLFLRSDALLAALAARAVKRPVKVMLPRPTIPNNTTHRPATLQHLRIGADQSGKIT AISHESWSGNLPGGTPETAVQQSELLYAGANRHTGLRLATLDLPEGNAMRAPGEAPGLMA LEIAIDELAEKAGIDPVEFRILNDTQVDPADPTRRFSRRQLIECLRTGADKFGWKQRNAT PGQVRDGEWLVGHGVAAGFRNNLLEKSGARVHLEPNGTVTVETDMTDIGTGSYTILAQTA AEMLGVPLEQVAVHLGDSSFPVSAGSGGQWGANTSTSGVYAACVKLREMIASAVGFDPEQ SQFADGKITNGTRSAMLHEATAGGRLIAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATG EVRVRRMLAVCAAGRILNPKTARSQVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYE VPVHADIPKQEVIFLDDTDPISSPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPIT LDKLLDKLPDVV