Protein Info for DZA65_RS22335 in Dickeya dianthicola ME23

Annotation: low affinity potassium transporter Kup

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 622 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details transmembrane" amino acids 49 to 69 (21 residues), see Phobius details amino acids 98 to 120 (23 residues), see Phobius details amino acids 135 to 156 (22 residues), see Phobius details amino acids 168 to 189 (22 residues), see Phobius details amino acids 210 to 233 (24 residues), see Phobius details amino acids 245 to 265 (21 residues), see Phobius details amino acids 285 to 311 (27 residues), see Phobius details amino acids 337 to 357 (21 residues), see Phobius details amino acids 363 to 386 (24 residues), see Phobius details amino acids 395 to 413 (19 residues), see Phobius details amino acids 420 to 440 (21 residues), see Phobius details PF02705: K_trans" amino acids 13 to 462 (450 residues), 623.8 bits, see alignment E=1.4e-191 TIGR00794: potassium uptake protein" amino acids 95 to 550 (456 residues), 615.5 bits, see alignment E=6.6e-189 PF22776: K_trans_C" amino acids 475 to 622 (148 residues), 166.1 bits, see alignment E=5e-53

Best Hits

Swiss-Prot: 89% identical to KUP_PECCP: Low affinity potassium transport system protein kup (kup) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K03549, KUP system potassium uptake protein (inferred from 98% identity to ddc:Dd586_4145)

MetaCyc: 82% identical to K+:H+ symporter Kup (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-3

Predicted SEED Role

"Kup system potassium uptake protein" in subsystem Potassium homeostasis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CXS3 at UniProt or InterPro

Protein Sequence (622 amino acids)

>DZA65_RS22335 low affinity potassium transporter Kup (Dickeya dianthicola ME23)
MNSEHKRSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGVEPDSVFGFLSLIFWLL
VLVVSLKYLSYVMRADNAGEGGILTLMSLAGRNTSDKVTAIVVIMGLIGGSFFYGEVVIT
PAISVMSAIEGLDIVAPSLDTYIVPISIVVLTLLFMIQKHGTGRVGSLFAPIMMLWFLSL
GVLGARSIIANPEVLQALNPKWAINFFIQYKAVSFFALGAVVLAITGVEALYADMGHFGK
LPIRIAWFSAVLPSLVLNYFGQGALLLKNPDAIKNPFFLLAPDWALIPLLVLATLATIIA
SQAVISGVFSLTRQAVRLGYLPPMRIVHTSDMESGQIYIPFINWLLYVAVVIVIVSFEHS
SNLAAAYGIAVTGTMVLTSILSCTVALKNWHWYRYLVWLLLVALLAIDLPMFLANVVKII
SGGWLPLALGMVMFTIMTTWKSERFRLLRRIHEHGNSLDAMIASLEKNPPVRVTGTAVYL
SRATHVIPFALLHNLKHNKVLHERVVLLTMRTEDAPYVHNARRVSVEQLSPTFWRVVASY
GWRETPNVEEVFHRCWQDGLTCQMMETTFFLSNESLMMGNRPLYLRVRGKLFMMLSRNAL
RAADQFEIPPNRLIELGIQVEI