Protein Info for DZA65_RS21445 in Dickeya dianthicola ME23

Annotation: Si-specific NAD(P)(+) transhydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 466 PF07992: Pyr_redox_2" amino acids 7 to 326 (320 residues), 211.7 bits, see alignment E=6.4e-66 PF00890: FAD_binding_2" amino acids 8 to 48 (41 residues), 24.3 bits, see alignment 7.1e-09 PF12831: FAD_oxidored" amino acids 8 to 55 (48 residues), 30.2 bits, see alignment 1.3e-10 PF13450: NAD_binding_8" amino acids 11 to 67 (57 residues), 22.5 bits, see alignment 4.6e-08 PF00070: Pyr_redox" amino acids 178 to 254 (77 residues), 56.1 bits, see alignment E=1.8e-18 PF02852: Pyr_redox_dim" amino acids 346 to 457 (112 residues), 94.4 bits, see alignment E=2.1e-30

Best Hits

Swiss-Prot: 92% identical to STHA_PECAS: Soluble pyridine nucleotide transhydrogenase (sthA) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K00322, NAD(P) transhydrogenase [EC: 1.6.1.1] (inferred from 98% identity to ddd:Dda3937_03893)

MetaCyc: 86% identical to soluble pyridine nucleotide transhydrogenase (Escherichia coli K-12 substr. MG1655)
NAD(P)H oxidase. [EC: 1.6.3.1]; 1.6.3.- [EC: 1.6.3.1]; NAD(P)(+) transhydrogenase (B-specific). [EC: 1.6.3.1, 1.6.1.1, 1.6.1.3]

Predicted SEED Role

"Soluble pyridine nucleotide transhydrogenase (EC 1.6.1.1)" in subsystem Phosphate metabolism (EC 1.6.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.6.1.1 or 1.6.1.3 or 1.6.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385Y3I7 at UniProt or InterPro

Protein Sequence (466 amino acids)

>DZA65_RS21445 Si-specific NAD(P)(+) transhydrogenase (Dickeya dianthicola ME23)
MQQQYDYDAIVIGSGPGGEGAAMGLAKQGAKIAVIERYYNVGGGCTHWGTIPSKALRHAV
SRIIEFNQNPLYSDNARVISSSFSDILRHADSVIGQQTRMRQGFYERNHCELFSGEARFI
DAHTIAVYYPDDTHDTLTAANIIIATGSRPYHPAGVDFDHPRIYDSDSILDLDYEPKHVI
IYGAGVIGCEYASIFRGLNVKVDLINTRDRLLAFLDQEMSDALSYHFWNSGVVIRHNEEF
ECIEGLDDGVIIHLKSGKKMKADCLLYANGRTGNTETLELENIGLETDSRGLLKVNSMYQ
TAQAHIYAVGDVIGYPSLASAAYDQGRIAAQAITKGDATAHLIEDIPTGIYTIPEISSVG
KTEQELTAMKVPYEVGRAQFKHLARAQIVGMNVGSLKILFHRDTKQILGIHCFGERAAEI
IHIGQAIMEQKGEGNTIEYFVNTTFNYPTMAEAYRVAALNGLNRLF