Protein Info for DZA65_RS21300 in Dickeya dianthicola ME23
Annotation: trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 76% identical to PUTA_ECOLI: Bifunctional protein PutA (putA) from Escherichia coli (strain K12)
MetaCyc: 76% identical to fused DNA-binding transcriptional repressor / proline dehydrogenase / 1-pyrroline-5-carboxylate dehydrogenase PutA (Escherichia coli K-12 substr. MG1655)
RXN0-7008 [EC: 1.5.5.2]; RXN-14116 [EC: 1.5.5.2, 1.2.1.88]
Predicted SEED Role
"Transcriptional repressor of PutA and PutP / Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)" in subsystem Arginine and Ornithine Degradation or Proline, 4-hydroxyproline uptake and utilization or Respiratory dehydrogenases 1 (EC 1.5.1.12, EC 1.5.99.8)
MetaCyc Pathways
- L-proline degradation I (2/3 steps found)
- proline to cytochrome bo oxidase electron transfer (1/2 steps found)
- L-citrulline biosynthesis (5/8 steps found)
- L-arginine degradation I (arginase pathway) (1/3 steps found)
- superpathway of L-citrulline metabolism (7/12 steps found)
- ethene biosynthesis II (microbes) (1/4 steps found)
- L-Nδ-acetylornithine biosynthesis (3/7 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.2.1.88 or 1.5.1.12 or 1.5.5.2 or 1.5.99.8
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A385Y3G6 at UniProt or InterPro
Protein Sequence (1325 amino acids)
>DZA65_RS21300 trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase (Dickeya dianthicola ME23) MGTTTMGVKLDEATRERLKAVAQRIDRTPHWLIKQAIFSYLERLESGADTPEIPHPVGQD LIEAAEIMPQLPQEETHQPFLNFAEQVLPQSVLRSAITAAYRRPETEMVPMLLEQARMPG AVSHAASALAAGLAEKLRRQKNSGGRAGMVQSLLQEFSLSSQEGVALMCLAEALLRIPDK PTRDALIRDKISTGNWQSHVGRSASLFVNAAAWGLLVTGKLVSTHNESHLSGALNRIISK SGEPLVRKGVDMAMRLMGEQFVAGETIAEALANARKREERGFRYSYDMLGEAALTGDDAE AYLTAYQQAIHAIGKAAGGRGIYEGPGISIKLSALHPRYSRAQYERVMDELYPRLLALTL LARQYDIGINIDAEEADRLELSLDLLERLCFEPRLAGWNGIGFVIQAYQKRCPFVIDALV DLARRSQRRLMIRLVKGAYWDSEIKRAQMDGLEGYPVYTRKLYTDVSYLACARRLLAAPN LIYPQFATHNAHTLSAIYHLAGNNYYPGQYEFQCLHGMGEPLYEQVVGKAADGKLNRPCR IYAPVGTHETLLAYLVRRLLENGANTSFVNRIADPSVALEALVADPVSETEALAARDGLV GQPHPRIPLPRALYGGTRRNAGGLDLSSEHRLASLSSALLTSAARSWQAQPMLAVEGDSG GDSRPVINPAEQRDVVGYVREAQPQDIAQALEQAASAGDIWFATPAAERAAILSRAADLL ESRQQHLLGLLVREAGKTLANAVAEVREAVDFLRYYAAQVSETFNNHDYRPLGPVVCISP WNFPLAIFTGQIAAALAAGNSVLAKPAEQTPLIAAQAVQMLHEAGVPAGALQLLPGAGET VGAALVRDERVRGVMFTGSTVVAAQLQRMLAGRLDPQGRTTPLIAETGGINAMIVDSSAL TEQVVADVVTSAFDSAGQRCSALRLLCVQEEVAEKTLAMLRGAIAECRMGNPERLSTDIG PLIDAEAKQRIARHIQTLRTKGRPVFQAAWPNSQDQQEWARGHFIPPTLIELESVGDLQQ EVFGPVLHVVRYSRNQLDALIGHINAAGYGLTLGLHTRIDETINRVTSQATVGNLYVNRN MVGAVVGVQPFGGEGLSGTGPKAGGPLYLHRLLSHRPQDAAIRTLARQDRERPADVAARQ PLLAGLQALASWARQVQRDVLVSCCQRYGELTQSGTVRVLPGPTGERDTYTLLPREQVLC VADNDDDALVQLAAVLAVGSRALWVEAPERQALYRQLPEAVQTRVAFCRDGVTDDSRFDA VIFHGDADRLRHLSEQLAQRDGAIVGVQGLSRGETDIVLERLLIERSLSINTAAAGGNAS LMTIG