Protein Info for DZA65_RS20175 in Dickeya dianthicola ME23

Annotation: elongation factor P--(R)-beta-lysine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 325 PF00152: tRNA-synt_2" amino acids 14 to 322 (309 residues), 182.5 bits, see alignment E=5.5e-58 TIGR00462: EF-P lysine aminoacylase GenX" amino acids 17 to 320 (304 residues), 442.4 bits, see alignment E=4.8e-137

Best Hits

Swiss-Prot: 85% identical to EPMA_PECCP: Elongation factor P--(R)-beta-lysine ligase (epmA) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K04568, lysyl-tRNA synthetase, class II [EC: 6.1.1.6] (inferred from 98% identity to ddd:Dda3937_02148)

MetaCyc: 79% identical to EF-P-lysine lysyltransferase (Escherichia coli K-12 substr. MG1655)
6.3.2.-

Predicted SEED Role

"Translation elongation factor P Lys34:lysine transferase"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.6

Use Curated BLAST to search for 6.1.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385Y5Y3 at UniProt or InterPro

Protein Sequence (325 amino acids)

>DZA65_RS20175 elongation factor P--(R)-beta-lysine ligase (Dickeya dianthicola ME23)
MSETASWQPSAPVANLLKRASIMKEIRRFFSDRGVLEVETPAMSQATVTDVHLFPFQTHF
VGPGAADGMALYLMTSPEYHMKRLLAAGSGPIYQLCRSFRNEESGRYHNPEFTMLEWYRP
HYDMYRLMNEMDDLLQQVLECDSAEMLSYQQAFQRHLEVDPLSADKAQLREAAEKIGVGD
VAGREEDRDTLLQLLFVFGVEPHIGRDKPAFVYHFPATQAALAEISTEDHRVAERFEVYY
KGIELANGFCELTDSKEQRQRFEQDNRKRAARSLPVQPIDENLLTALEAGIPPCSGVALG
VDRLVMLALNAESISEVMAFTVDRA