Protein Info for DZA65_RS19975 in Dickeya dianthicola ME23

Annotation: nitrogen fixation protein NifX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 155 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF02579: Nitro_FeMo-Co" amino acids 28 to 119 (92 residues), 57 bits, see alignment E=9.8e-20

Best Hits

Swiss-Prot: 67% identical to NIFX_KLEPN: Protein NifX (nifX) from Klebsiella pneumoniae

KEGG orthology group: K02596, nitrogen fixation protein NifX (inferred from 96% identity to ddd:Dda3937_02166)

Predicted SEED Role

"Nitrogenase FeMo-cofactor carrier protein NifX" in subsystem Nitrogen fixation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CAM7 at UniProt or InterPro

Protein Sequence (155 amino acids)

>DZA65_RS19975 nitrogen fixation protein NifX (Dickeya dianthicola ME23)
MQHVNRHLSAISAGVWSMKVAFASSDYHHVDQHFGATPRLVIYGVKEDQVTLLKVADFNV
RHGHQADKLTCRIDALEDCVTLYCVAIGDAVFRQLLQVGVRAVRVPADTPIAQLLQQIQQ
CWHDDEQRVARRQRDPDRFERLIAEQEWSEEDDVL