Protein Info for DZA65_RS19380 in Dickeya dianthicola ME23
Annotation: carbamoyl-phosphate synthase large subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 93% identical to CARB_ECOLI: Carbamoyl-phosphate synthase large chain (carB) from Escherichia coli (strain K12)
KEGG orthology group: K01955, carbamoyl-phosphate synthase large subunit [EC: 6.3.5.5] (inferred from 74% identity to abo:ABO_0318)MetaCyc: 93% identical to carbamoyl-phosphate synthetase large subunit (Escherichia coli K-12 substr. MG1655)
Carbamoyl-phosphate synthase (glutamine-hydrolyzing). [EC: 6.3.5.5]
Predicted SEED Role
"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
- superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
- L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
- superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
- L-arginine biosynthesis II (acetyl cycle) (9/10 steps found)
- superpathway of arginine and polyamine biosynthesis (14/17 steps found)
- UMP biosynthesis I (6/6 steps found)
- UMP biosynthesis II (6/6 steps found)
- L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
- L-arginine degradation V (arginine deiminase pathway) (4/4 steps found)
- L-citrulline degradation (3/3 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- UMP biosynthesis III (5/6 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (3/4 steps found)
- L-glutamate and L-glutamine biosynthesis (5/7 steps found)
- L-citrulline biosynthesis (5/8 steps found)
- superpathway of L-citrulline metabolism (7/12 steps found)
- L-arginine biosynthesis IV (archaea) (4/9 steps found)
- allantoin degradation IV (anaerobic) (3/9 steps found)
- guadinomine B biosynthesis (2/13 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.5.5
Use Curated BLAST to search for 6.3.5.5
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A3A4C8P4 at UniProt or InterPro
Protein Sequence (1074 amino acids)
>DZA65_RS19380 carbamoyl-phosphate synthase large subunit (Dickeya dianthicola ME23) MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRVVLVNSNPATIMTDPEM ADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALELERQGVLAEFGVTMIGATA DAIDKAEDRRRFDIAMKKIGLETARSGIAHNMDEALAVAADVGYPCIIRPSFTMGGTGGG IAYNREEFEEICTRGLDLSPTKELLIDESLIGWKEYEMEVVRDKNDNCIIVCSIENLDAM GIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIGVETGGSNVQFAVNPKTGRLIVIEM NPRVSRSSALASKATGFPIAKIAAKLAVGYTLDELMNDITGGRTPASFEPSIDYVVTKIP RFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQESLQKALRGLEVGATGFDPKVELDDPEA LTKIRRELKDAGGDRIWYLADAFRAGMSVDGVFNLTNIDRWFLVQIEELVRLEEQVAEQG ANGLTQEFLRLLKRKGFADARLAALAGVSEGEIRKLRYKYDLHPVYKRVDTCAAEFATDT AYMYSTYDEECEANPNQDRDKIMVLGGGPNRIGQGIEFDYCCVHAALALREDGYETIMVN CNPETVSTDYDTSDRLYFEPVTLEDVLEIVRIEQPKGVIVQYGGQTPLKLARQLEAAGVP VIGTSPDAIDRAEDRERFQQAVNRLGLKQPANATVSTIDQAVEKAVGIGYPLVVRPSYVL GGRAMEIVSDEIDLRRYFQTAVSVSNDAPVLLDRFLDDAVEVDVDAICDGERVLIGGIME HIEQAGVHSGDSACSLPAYTLSKDIQDVMRQQVEKLAFELSVRGLMNVQFAVKNNEVYLI EVNPRAARTVPFVSKATGVPLAKVAARVMVGQTLAQQGVTKEVIPPYYSVKEVVLPFNKF PGVDPILGPEMRSTGEVMGVGRTFAEAFAKAMLGSNSPMKKTGRALLSVREGDKARVVDL AAKLLKHGFELDATHGTAVVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIVNTTAGR QAIEDSKLIRRSALQYKVHYDTTMNGGFATAMSLNADPTEQVVSVQEMHARIQG