Protein Info for DZA65_RS17145 in Dickeya dianthicola ME23

Annotation: polynucleotide adenylyltransferase PcnB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 467 TIGR01942: poly(A) polymerase" amino acids 31 to 461 (431 residues), 527.9 bits, see alignment E=8.8e-163 PF01743: PolyA_pol" amino acids 62 to 195 (134 residues), 140.3 bits, see alignment E=6.7e-45 PF12627: PolyA_pol_RNAbd" amino acids 223 to 282 (60 residues), 70.1 bits, see alignment E=1.6e-23 PF12626: PolyA_pol_arg_C" amino acids 340 to 459 (120 residues), 139.1 bits, see alignment E=1.1e-44

Best Hits

Swiss-Prot: 79% identical to PCNB_ECOLI: Poly(A) polymerase I (pcnB) from Escherichia coli (strain K12)

KEGG orthology group: K00970, poly(A) polymerase [EC: 2.7.7.19] (inferred from 98% identity to ddd:Dda3937_00513)

MetaCyc: 79% identical to poly(A) polymerase I (Escherichia coli K-12 substr. MG1655)
Polynucleotide adenylyltransferase. [EC: 2.7.7.19]

Predicted SEED Role

"Poly(A) polymerase (EC 2.7.7.19)" in subsystem Polyadenylation bacterial (EC 2.7.7.19)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385Y0P1 at UniProt or InterPro

Protein Sequence (467 amino acids)

>DZA65_RS17145 polynucleotide adenylyltransferase PcnB (Dickeya dianthicola ME23)
MFTRVANFCRKVLNRENEPARADNPPQAMTVVPRDQHAISRSDISENALKVLYRLSKAGF
EAYLVGGGVRDLLLGKKPKDFDITTNATPEQVRKLFRNCRLVGRRFRLAHVMFGPEVIEV
ATFRGHHEQHQEQETKNAAQQGHNGMLLRDNIFGSIEEDAQRRDFSINSLYYSIADFTVR
DYTNGLNDLRQRLIRLIGEPETRYREDPVRMLRAVRFAAKLSMRISPETAEPIPRLAALL
HDIPPARLFEESLKLLQAGYGYPTYQLLCEYQLFQPLFPLISRYFTANGESTMERMIVQV
LKNTDQRIQNDMRVNPAFLFSAMLWYPLVEHAQKLTQESGLAYFEAFALAMNDVLDEQCR
SLAIPKRITSLTRDIWQLQLRLSRRQGKRAFKLMEHPKFRAAYDLLCLRAEIENHQELQR
LAQWWGEFQVAAPPRQQVLLNTLDDGPTPHRRSRRPRKRPDRRDNAR