Protein Info for DZA65_RS17120 in Dickeya dianthicola ME23

Annotation: ATP-dependent helicase HrpB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 823 TIGR01970: ATP-dependent helicase HrpB" amino acids 5 to 820 (816 residues), 1099.3 bits, see alignment E=0 PF00270: DEAD" amino acids 12 to 162 (151 residues), 43.7 bits, see alignment E=5.2e-15 PF00271: Helicase_C" amino acids 203 to 327 (125 residues), 50.9 bits, see alignment E=3.4e-17 PF24473: CON_HrpB" amino acids 578 to 612 (35 residues), 45.8 bits, see alignment (E = 8.4e-16) PF08482: HrpB_C" amino acids 676 to 808 (133 residues), 174.1 bits, see alignment E=3.4e-55

Best Hits

KEGG orthology group: K03579, ATP-dependent helicase HrpB [EC: 3.6.4.13] (inferred from 94% identity to ddd:Dda3937_00507)

Predicted SEED Role

"ATP-dependent helicase HrpB"

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385Y0I3 at UniProt or InterPro

Protein Sequence (823 amino acids)

>DZA65_RS17120 ATP-dependent helicase HrpB (Dickeya dianthicola ME23)
MSLPPVSAVLDEVIRALHTAPQVLLHAPTGAGKSTWLPLQLLQRSGFPGRIIMLEPRRLA
AKSVAFRLAEWLGETPGQTVGYRMRSESRVSSQTRLEVVTEGILTRMLQHDPALDGVSLV
MLDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLDNSRLSSLLPQSLSVSSQGRS
WPVERHYQSVGSQERLEEATARLVYRLLQDETGSLLVFLPGVAEIKRLQTLLENAVPAEA
DICPLYGALTLAEQQKAILPAAAGRRKIVLATNIAETSLTIEGIRVVVDSGLERVVLFDV
KTGLTRLTTQRISQASMVQRAGRAGRLEPGACWHLFAREQAERAAAQSEPEILHSDLSGL
RLDLLQWGCNDVAQLCWLDTPPQPALLAADRLLAQLGMRDAQQRLTADGRKMAALGCDPR
LAAMLHAAGQDADVLATAALLAAIIEEPPRAGSPNLSDALHRPAGHWQRRAQQLARRVGK
ASGKVNADEVNAGKVNENLAAWLLSAGFADRIARRRGQDGRYQLANGSGASLPPEDALAA
SEWLLAPVLMQSPNNADARILLALPMDIDQLARQRPELVTEQNLMHWDEEKGTLRASLRT
QIGSLTLSSRPLTKPSDEALQQAMLNWLREQGLSVLNWDEPALQLRQRLLCARRWLPEAA
WPAVDDASLLASLERWLLPSLNGVRDRRTLHQINLSDALWRLLDWSQRQRLDSALPSHYT
APGGSRLPIRYDADLPPVLAVRLQEMFGEQRSPLLAEGRVALVLELLSPAMRPLQITRDL
AAFWQGAYREVQKEMKGRYPKHVWPDDPANTAPTRRTRKYQNS