Protein Info for DZA65_RS15510 in Dickeya dianthicola ME23

Annotation: malate dehydrogenase (quinone)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 76% identical to MQO_PECCP: Probable malate:quinone oxidoreductase (mqo) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K00116, malate dehydrogenase (quinone) [EC: 1.1.5.4] (inferred from 96% identity to ddd:Dda3937_00485)

MetaCyc: 68% identical to malate:quinone oxidoreductase (Escherichia coli K-12 substr. MG1655)
MALATE-DEHYDROGENASE-ACCEPTOR-RXN [EC: 1.1.5.4]

Predicted SEED Role

"Malate:quinone oxidoreductase (EC 1.1.5.4)" in subsystem TCA Cycle (EC 1.1.5.4)

MetaCyc Pathways

superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
superpathway of glyoxylate bypass and TCA (11/12 steps found)
TCA cycle I (prokaryotic) (9/10 steps found)
partial TCA cycle (obligate autotrophs) (7/8 steps found)
TCA cycle VI (Helicobacter) (7/9 steps found)
TCA cycle VII (acetate-producers) (6/9 steps found)

KEGG Metabolic Maps

Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.5.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CDF3 at UniProt or InterPro

Protein Sequence (523 amino acids)

>DZA65_RS15510 malate dehydrogenase (quinone) (Dickeya dianthicola ME23)
MKKRVVTTLCLGAIGLAQPVMAEENKAVDVVLIGGGVMSATLGTYLQVLEPNWTISMVER
LDNVAEESSNGWNNAGTGHSAFMELNYTPEKADGSIDISKAVEISEAFEISRQFWSYQIS
HNVMKDPSSFINSVPHMSFVWGDDNVNYLNKRYQALQHSTLFRGMEFSDNAEQIKQWIPL
VMEGRDPKQKIAATRMPIGTDVNYGEVTRQLVDGLKQHTTFNLKLSNEVRDIKRNPDKTW
SVVVADLKNGGKESAIKARFVFIGAGGAALQLLQKSGIPEAGNYGGFPVGGEFLVTDNQD
IVKRHLAKVYGKASVGAPPMSVPHLDTRIFNGKPALLFGPFATFSSKFLKNGSLWDLLSS
MNTSNFMPMVNVGIDNVDLVKYLIGQLMMSDDDRFAALKEYFPQAKQSDWRLAVAGQRVQ
VIKKDNDLGGVLKLGTEIVSDKDGSIAALLGASPGASTAAPIMLSLMQTVFKDKVATPEW
QAKLKEIVPSYGQKLEGNIDLTNKIRGYTSSTLKLNYMEVKPE