Protein Info for DZA65_RS14960 in Dickeya dianthicola ME23

Annotation: NADH-quinone oxidoreductase subunit NuoG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 908 PF13510: Fer2_4" amino acids 3 to 78 (76 residues), 44.5 bits, see alignment E=3.9e-15 PF00111: Fer2" amino acids 4 to 71 (68 residues), 31.6 bits, see alignment 4e-11 TIGR01973: NADH dehydrogenase (quinone), G subunit" amino acids 4 to 655 (652 residues), 673.5 bits, see alignment E=1.7e-206 PF10588: NADH-G_4Fe-4S_3" amino acids 88 to 125 (38 residues), 62.1 bits, see alignment (E = 8.4e-21) PF22117: Fer4_Nqo3" amino acids 141 to 207 (67 residues), 92.7 bits, see alignment 4.1e-30 PF04879: Molybdop_Fe4S4" amino acids 221 to 274 (54 residues), 47.1 bits, see alignment 5.5e-16 PF00384: Molybdopterin" amino acids 280 to 637 (358 residues), 46.9 bits, see alignment E=6.2e-16

Best Hits

Swiss-Prot: 82% identical to NUOG_SALTI: NADH-quinone oxidoreductase subunit G (nuoG) from Salmonella typhi

KEGG orthology group: K00336, NADH dehydrogenase I subunit G [EC: 1.6.5.3] (inferred from 96% identity to ddc:Dd586_2743)

MetaCyc: 66% identical to NADH-quinone oxidoreductase subunit G (Pseudomonas putida KT2440)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4D9N5 at UniProt or InterPro

Protein Sequence (908 amino acids)

>DZA65_RS14960 NADH-quinone oxidoreductase subunit NuoG (Dickeya dianthicola ME23)
MATIHVDGKEYEVDGADNLLQACLSLGLDIPYFCWHPALGSVGACRLCAVKQYQNAEDTR
GRLVMSCMTPASDGTYIAIDDDEAKQFRKTIVEFLMTNHPHDCPVCEEGGNCHLQDMTVM
AGHNFRRYRFTKRTHHNQDLGPFISHEMNRCIACYRCVRYYKDYADGKDLGVYGAHDNVY
FGRPEDGVLESEFSGNLVEVCPTGVFTDKTHSERYNRKWDMQFAPSICQQCSVGCNTSPG
ERYGELRRIENRYNGSVNHYFLCDRGRFGYGYVNLKDRPRQPLQRRGDDWIALNAEQALQ
GAADVLRQAKRVIGIGSPRASVESNFALRELVGAGNFYTGVSQAEQHRLQLMLKVLKESG
VHTPSLREIEGYDAVLVLGEDLTQTGARIALAVRQAVKGKAREMAAAQKVADWQIAAILN
IGQHAKHPLFVTNVDSTRLDDIAAWSYRAPVAEQARLGFAIAHALDNAAPAVNDLPADLS
QKVDVIVQALAGARKPLIISGTNAGSDDVIAAAANVAKALKGRGADVGITYVAASANSMG
VTMMGGGSLDEALSQLETGEADSVVVLENDLYRHAPAARVDAALAKAANLIVLDHQRTAI
MDKASLVLSSASFAESGGTLVSQEGRAQRFFQVYDPTYYDSKVVMLESWRWLHSLYITYN
SRQVDWTQLDHVIDACVNALPQLAAIKDAAPDASFRINGQKLAREPHRYSGRTAMRANIS
VHEPRQPQDRDTMFTFSMEGNNSPLAERQQVPFAWAPGWNSPQAWNKFQDEVGGHLRHGD
PGVRMIEAGDGSLTYFSRIPAPFAPQQGQWQVAPYYNLFGSEELSQRSGVIQQRMPPACA
VVNQADAALLGVNAGALLDITCGGQTLRLPVRLSTELRQGQVGLPLGFPGIAPVLAGATV
EMLREAVQ