Protein Info for DZA65_RS14795 in Dickeya dianthicola ME23

Annotation: multidrug effflux MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 43 to 66 (24 residues), see Phobius details amino acids 78 to 97 (20 residues), see Phobius details amino acids 103 to 124 (22 residues), see Phobius details amino acids 136 to 160 (25 residues), see Phobius details amino acids 166 to 186 (21 residues), see Phobius details amino acids 216 to 236 (21 residues), see Phobius details amino acids 250 to 270 (21 residues), see Phobius details amino acids 282 to 303 (22 residues), see Phobius details amino acids 309 to 332 (24 residues), see Phobius details amino acids 343 to 364 (22 residues), see Phobius details amino acids 370 to 391 (22 residues), see Phobius details TIGR00710: drug resistance transporter, Bcr/CflA subfamily" amino acids 9 to 391 (383 residues), 298.1 bits, see alignment E=6.8e-93 PF07690: MFS_1" amino acids 22 to 357 (336 residues), 152.7 bits, see alignment E=1.3e-48 PF00083: Sugar_tr" amino acids 38 to 118 (81 residues), 33.3 bits, see alignment E=2.6e-12

Best Hits

Swiss-Prot: 43% identical to YDGK_BACSU: Uncharacterized MFS-type transporter YdgK (ydgK) from Bacillus subtilis (strain 168)

KEGG orthology group: K07552, MFS transporter, DHA1 family, bicyclomycin/chloramphenicol resistance protein (inferred from 92% identity to ddd:Dda3937_01837)

Predicted SEED Role

"Bicyclomycin resistance protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4DN37 at UniProt or InterPro

Protein Sequence (400 amino acids)

>DZA65_RS14795 multidrug effflux MFS transporter (Dickeya dianthicola ME23)
MTEKLSRSQLEYALILGSLAALGPLCIDLYLPALPQMTGALAASTAVTQLSLTAGLLGLG
AGQLIFGPLSDKLGRRRPLLLSLTMLLLTSVWCALAQDIGQLIVARLLQGIAGAGGAVLS
RAIARDLYVGHALTRFFSLLMLINGLAPILSPVLGGVLLGVTDWRGIFVLLAVIACLLLT
MSVLRLNETLPAERRIAGGVGSMLSSLGSLLRERTFMGLCLAQGLCGAGMFAYIGASPFV
LQEVYGLSPQAFSLCFAANGIGLIAAGQLASHFSQRFGEQRVLLTGLIIAVISALALTAA
GLLHAPLIGILIPLFFAIAMIGIVGPCASSLAMQSQGNKAGSASALLGLNMFLFGALSVP
LTGLAGSTSVLSMALVILGCYLLAALAYRMLSSQPLVRKA