Protein Info for DZA65_RS12480 in Dickeya dianthicola ME23

Annotation: NarK family nitrate/nitrite MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 463 transmembrane" amino acids 39 to 62 (24 residues), see Phobius details amino acids 75 to 93 (19 residues), see Phobius details amino acids 105 to 124 (20 residues), see Phobius details amino acids 131 to 155 (25 residues), see Phobius details amino acids 173 to 196 (24 residues), see Phobius details amino acids 216 to 234 (19 residues), see Phobius details amino acids 255 to 278 (24 residues), see Phobius details amino acids 289 to 308 (20 residues), see Phobius details amino acids 319 to 339 (21 residues), see Phobius details amino acids 348 to 375 (28 residues), see Phobius details amino acids 404 to 425 (22 residues), see Phobius details amino acids 437 to 457 (21 residues), see Phobius details TIGR00886: nitrite transporter" amino acids 37 to 418 (382 residues), 357.1 bits, see alignment E=5.5e-111 PF07690: MFS_1" amino acids 46 to 376 (331 residues), 60.2 bits, see alignment E=8.4e-21

Best Hits

Swiss-Prot: 73% identical to NARK_ECOLI: Nitrate/nitrite transporter NarK (narK) from Escherichia coli (strain K12)

KEGG orthology group: K02575, MFS transporter, NNP family, nitrate/nitrite transporter (inferred from 97% identity to ddd:Dda3937_03788)

MetaCyc: 73% identical to nitrate:nitrite antiporter NarK (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-239

Predicted SEED Role

"Nitrate/nitrite transporter" in subsystem Nitrate and nitrite ammonification

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CHW6 at UniProt or InterPro

Protein Sequence (463 amino acids)

>DZA65_RS12480 NarK family nitrate/nitrite MFS transporter (Dickeya dianthicola ME23)
MTQSTTTPGASRQTVIRDWRPEDVQFWQKQGRGIAQRNLWISIPCLLLAFCVWMLFSTVA
VNLNKVGFHFTTEQLFMLTALPSVSGALLRVPYSFVIPLAGGRRWTTFSTLILIIPCVWL
GFVVQDPQTPYSTFIIISLLCGFAGANFASSMANISFFFPKARQGGALGLNGGFGNLGVS
VMQLLVPVVIFLPVLSFTGKGVAQPDGGQVWLQNAPWVWVPLLLIASAAAWFGMNDLSTA
KASLRQQLPVLKRAHLWILSVLYLSAFGSFIGFSAGFAMLSKTQFPDVIILHYAFCGPLL
GALARPVGGMLSDRFGGVRVTLINFVFMAILSVLLFTALPGDNQPGSFLLFFGIFMLLFL
TAGLGSGSTFQMIAVIFRGLTVERVTSAGGGEDEAQRSAVTDTAAALGFISAIGAIGGFF
IPKAFGTSLAMTGSPAGAMKIFVVFYIACVFITWLCYGRKSRS