Protein Info for DZA65_RS10770 in Dickeya dianthicola ME23

Annotation: asparaginase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 82% identical to ASPG1_ECO57: L-asparaginase 1 (ansA) from Escherichia coli O157:H7

KEGG orthology group: K01424, L-asparaginase [EC: 3.5.1.1] (inferred from 98% identity to ddd:Dda3937_04331)

MetaCyc: 82% identical to L-asparaginase 1 (Escherichia coli K-12 substr. MG1655)
Asparaginase. [EC: 3.5.1.1, 3.5.1.38, 3.5.5.4]

Predicted SEED Role

"L-asparaginase (EC 3.5.1.1)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 3.5.1.1)

MetaCyc Pathways

superpathway of L-aspartate and L-asparagine biosynthesis (4/4 steps found)
ammonia assimilation cycle III (3/3 steps found)
L-glutamate biosynthesis I (2/2 steps found)
L-asparagine degradation I (1/1 steps found)
L-glutamine degradation I (1/1 steps found)
L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
L-glutamate and L-glutamine biosynthesis (5/7 steps found)
L-asparagine degradation III (mammalian) (2/3 steps found)
L-citrulline biosynthesis (5/8 steps found)
N-(1-deoxy-D-fructos-1-yl)-L-asparagine degradation (2/5 steps found)
superpathway of L-citrulline metabolism (7/12 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.1

Use Curated BLAST to search for 3.5.1.1 or 3.5.1.38 or 3.5.5.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XXW8 at UniProt or InterPro

Protein Sequence (339 amino acids)

>DZA65_RS10770 asparaginase (Dickeya dianthicola ME23)
MQKKSIYVAYTGGTIGMQRSAHGYIPVSGHLQQQLAQMPEFHRPEMPSFTIHEYIPLIDS
SDMTPSDWQSIANDIQQHYDQYDGFVILHGTDTMAFTASALSFMLENLSKPVIVTGSQIP
LAELRSDGQTNLLNALYVAANHPVNEVSLFFNNKLLRGNRTTKAHADGFDAFASPNFPPL
LEAGIHIRRLTPANPDPISGPLVVHAITPQPIGVVTIYPGISADVVSNFLLQPVKALILR
SYGVGNAPQSSGLLDELRRASERGIVVVNLTQCISGRVNMDGYATGNALAQAGVISGFDM
TVEAALTKLHYLLSRNDLSPDQIRQLMQQNLRGELSDKD