Protein Info for DZA65_RS09780 in Dickeya dianthicola ME23

Annotation: 30S ribosomal protein S6--L-glutamate ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 PF18030: Rimk_N" amino acids 1 to 93 (93 residues), 138.2 bits, see alignment E=2.8e-44 TIGR00768: alpha-L-glutamate ligase, RimK family" amino acids 2 to 286 (285 residues), 305.2 bits, see alignment E=2.1e-95 PF02655: ATP-grasp_3" amino acids 97 to 263 (167 residues), 35.8 bits, see alignment E=2.6e-12 PF08443: RimK" amino acids 97 to 286 (190 residues), 248.7 bits, see alignment E=9.9e-78 PF02955: GSH-S_ATP" amino acids 138 to 265 (128 residues), 58.6 bits, see alignment E=1.7e-19

Best Hits

Swiss-Prot: 86% identical to RIMK_PECCP: Probable alpha-L-glutamate ligase (rimK) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K05844, ribosomal protein S6 modification protein (inferred from 98% identity to ddd:Dda3937_03479)

MetaCyc: 78% identical to ribosomal protein S6 modification protein (Escherichia coli K-12 substr. MG1655)
6.3.2.-

Predicted SEED Role

"Ribosomal protein S6 glutaminyl transferase" in subsystem Ribosome biogenesis bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XWN9 at UniProt or InterPro

Protein Sequence (300 amino acids)

>DZA65_RS09780 30S ribosomal protein S6--L-glutamate ligase (Dickeya dianthicola ME23)
MKIAILSQDSSLYSCKRLCEAAEERQHNIEVINPLSCYMNINSAAPSVHYRGRQLDPYDA
VIPRIGAAITFYGTAVLRQFEMLGSVVLNNAMSVIRARDKLHSLQLLARQGIDLPITGFA
HSPDDTADLIKMVGGAPLVVKLVEGTQGIGVVLAETHQAAESVIDAFRGLNAHILVQEYV
REAHGKDIRCLVVGDRVVAAIERQAKPGDFRSNLHRGGSASRVRITPQERAIAVKAAKTL
GLNVAGVDILRSARGPLVMEVNASPGLEGIETTTGLDIAGMMIEYIEHHHRAVSRSVRAG