Protein Info for DZA65_RS09245 in Dickeya dianthicola ME23

Annotation: acetyl-CoA carboxylase biotin carboxylase subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00289: Biotin_carb_N
  • amino acids 5 to 113 (109 residues), 142.2 bits, E=2.2e-45
• PF02786: CPSase_L_D2
  • amino acids 118 to 324 (207 residues), 234.1 bits, E=3.2e-73
• PF02222: ATP-grasp
  • amino acids 127 to 294 (168 residues), 34.1 bits, E=5.3e-12
• PF07478: Dala_Dala_lig_C
  • amino acids 144 to 293 (150 residues), 27.4 bits, E=5.9e-10
• PF02785: Biotin_carb_C
  • amino acids 338 to 444 (107 residues), 122.1 bits, E=2.7e-39

Best Hits

Swiss-Prot: 52% identical to ACCC_PSEAE: Biotin carboxylase (accC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01961, acetyl-CoA carboxylase, biotin carboxylase subunit [EC: 6.3.4.14 6.4.1.2] (inferred from 94% identity to ddd:Dda3937_02461)

MetaCyc: 50% identical to 4-hydroxyacetophenone carboxylase biotin carboxylase subunit (Aromatoleum aromaticum EbN1)
RXN-9833

Predicted SEED Role

"Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)" in subsystem Fatty Acid Biosynthesis FASII (EC 6.3.4.14)

MetaCyc Pathways

• biotin-carboxyl carrier protein assembly (4/4 steps found)
• fatty acid biosynthesis initiation (type I) (1/3 steps found)
• 4-ethylphenol degradation (anaerobic) (2/6 steps found)
• 3-hydroxypropanoate cycle (6/13 steps found)
• jadomycin biosynthesis (3/9 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (9/18 steps found)
• glyoxylate assimilation (5/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (6/18 steps found)
• superpathway of mycolate biosynthesis (21/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis
• Propanoate metabolism
• Pyruvate metabolism
• Tetracycline biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14, 6.4.1.2

Use Curated BLAST to search for 6.3.4.14 or 6.4.1.2

Sequence Analysis Tools

See A0A385XWL9 at UniProt or InterPro

Protein Sequence (461 amino acids)

>DZA65_RS09245 acetyl-CoA carboxylase biotin carboxylase subunit (Dickeya dianthicola ME23)
MRQPIRKLLIANRGEIAVRIIHAARSLGIATVAACSEADVDSLPARLADETVLLGPARAD
QSYLNQAALLQAASDSGADAVHPGYGFLSENAAFAEAVEQAGLVFVGPTAQTIRMMGDKA
AARRTAQAAGVPVVPGSGVLSSLDAALQAAAEIGYPLLVKASAGGGGRGIRVVNHEDDLK
REFPIAQSEARAAFGCGDVYLELFIRHARHIEVQILGDGERAVHLYERECSLQRRRQKVF
EEAPSPALTPAQREALCDSALQLAQQLRYRSAGTLEYLFDAEREQFYFIEMNTRIQVEHP
VTERVTGVDLVQWMLRIAGGEPLSLRQEAIGLHGHACEMRINAEDPARNFFPCPGVVGTL
TWPQGPGIRIDSHLFSGYRIPPYYDSLLAKLVVSGADRTQALARAEQALRQLHIGGVTTT
QSLHQWLLADPRLRAGRFDTTSLESWLQTREAETLPLTKEA