Protein Info for DZA65_RS08315 in Dickeya dianthicola ME23

Annotation: isopenicillin N synthase family oxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 357 signal peptide" amino acids 1 to 16 (16 residues), see Phobius details PF14226: DIOX_N" amino acids 20 to 143 (124 residues), 118.9 bits, see alignment E=2e-38 PF03171: 2OG-FeII_Oxy" amino acids 192 to 289 (98 residues), 80.3 bits, see alignment E=1.2e-26

Best Hits

KEGG orthology group: K06892, (no description) (inferred from 93% identity to ddd:Dda3937_03011)

Predicted SEED Role

"2-Oxobutyrate oxidase, putative" in subsystem Methionine Degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XY58 at UniProt or InterPro

Protein Sequence (357 amino acids)

>DZA65_RS08315 isopenicillin N synthase family oxygenase (Dickeya dianthicola ME23)
MSRHWLILLMESAMSQPVSLPVLDFSLLDGNARQRADFLQRLNHAARETGFFYLTHHGAA
PELQQRVQRLSRAFFALPEAEKQRVAMIHSPHFRGYNFAGAERTCSEPDWREQFDIGAER
PPLRLLSGDPAWRRLQGPNQWPASLPQLKIALLEWQQTLTRISLRLLRAFAEVLGLPVTA
FDALYGDKPSEHIKLIRYPGRVTADSRQGVGAHKDSGFLTLLLQDQQSGLQVEVEPDHWV
EAHPLPGSFVVNIGELLELATDGYLRATVHRVVSPPASQERLSVAFFLGAQLDAVVPVFP
LSPALAELAHGPASDPNNPLLRDVGWNYLKGRLRSHPEVAKRYYGDVLQAQRQAVTA