Protein Info for DZA65_RS08060 in Dickeya dianthicola ME23

Annotation: type I-C CRISPR-associated protein Cas5

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 220 TIGR02593: CRISPR-associated protein Cas5" amino acids 3 to 44 (42 residues), 48.2 bits, see alignment 7e-17 PF09704: Cas_Cas5d" amino acids 4 to 210 (207 residues), 125.4 bits, see alignment E=1.6e-40 TIGR01876: CRISPR-associated protein Cas5, subtype I-C/DVULG" amino acids 5 to 219 (215 residues), 205.3 bits, see alignment E=1.1e-64

Best Hits

Swiss-Prot: 52% identical to CAS5D_MANSM: CRISPR pre-crRNA endoribonuclease Cas5d (cas5d) from Mannheimia succiniciproducens (strain MBEL55E)

KEGG orthology group: None (inferred from 95% identity to ddd:Dda3937_02858)

Predicted SEED Role

"CRISPR-associated protein, Cas5d family"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XVS0 at UniProt or InterPro

Protein Sequence (220 amino acids)

>DZA65_RS08060 type I-C CRISPR-associated protein Cas5 (Dickeya dianthicola ME23)
MPFCLDVQGEFACFTRPEMKVERVSYDVITPSAARSIFDAILWKPAIAWRVTRIEVINPI
RWINLRRNEVGSVISSRVVTSTMKAGSGTLGLYIEDDRQQRAGLFLRDVHYRLHGEFTLT
AQAGRSDNAGKFAEMFRRRAQKGQCINQPYLGCREFAADVLLIDDGTPQPPALAQDRELG
WMLYDMDYQQAQNPTPRFFHAAMTQGVIDIPAPDSPEVVA