Protein Info for DZA65_RS07985 in Dickeya dianthicola ME23

Annotation: alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 383 TIGR02318: phosphonate metabolism protein PhnM" amino acids 8 to 383 (376 residues), 506.5 bits, see alignment E=2.1e-156 PF07969: Amidohydro_3" amino acids 245 to 383 (139 residues), 32.9 bits, see alignment E=5.2e-12 PF01979: Amidohydro_1" amino acids 264 to 381 (118 residues), 28.1 bits, see alignment E=1.3e-10

Best Hits

Swiss-Prot: 72% identical to PHNM_ECOLI: Alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase (phnM) from Escherichia coli (strain K12)

KEGG orthology group: K06162, PhnM protein (inferred from 95% identity to dze:Dd1591_2688)

MetaCyc: 72% identical to RPnTP hydrolase (Escherichia coli K-12 substr. MG1655)
RXN-17955 [EC: 3.6.1.63]; 3.6.1.63 [EC: 3.6.1.63]

Predicted SEED Role

"Metal-dependent hydrolase involved in phosphonate metabolism" in subsystem Alkylphosphonate utilization

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.1.63

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XWG4 at UniProt or InterPro

Protein Sequence (383 amino acids)

>DZA65_RS07985 alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase (Dickeya dianthicola ME23)
MSATTRIVNNVQLVLQDEVVSGSLAWRDGAIEAFSSTPSQLPQALDGEGGWLLPGLVELH
TDNLDKFFMPRPKVDWPAHSAMSSHDALIVSSGITTVLDAVAIGDVRDGGHRLDNLRRMI
DAIVHSQQLGVNRAQHLLHLRCELPHDTTLPLFEALMDIPEVALVSLMDHSPGQRQFATP
EKYREYYQGKYHLNDAEMDTFETEQRMLSERWSMPNRLAIAAHCRERGIALASHDDATAA
HVHESLAQGCVIAEFPTTEQAAQASHASGMKVLMGAPNIVRGGSHSGNVAASYLAEQGLL
DILSSDYYPASLLDAAFRLAADERNPFTLPQTVAMVSANPAQSVGLSDRGRIADGLRADL
VLAHHAHGQVRIRHVWAQGRQVF