Protein Info for DZA65_RS06910 in Dickeya dianthicola ME23

Annotation: Tol-Pal system protein TolB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 428 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR02800: Tol-Pal system beta propeller repeat protein TolB" amino acids 14 to 425 (412 residues), 513.5 bits, see alignment E=2.2e-158 PF04052: TolB_N" amino acids 23 to 120 (98 residues), 107.8 bits, see alignment E=7.6e-35 PF07676: PD40" amino acids 196 to 221 (26 residues), 21.5 bits, see alignment (E = 4.7e-08) amino acids 235 to 270 (36 residues), 36.4 bits, see alignment 9.5e-13 amino acids 280 to 313 (34 residues), 42 bits, see alignment 1.7e-14 amino acids 368 to 396 (29 residues), 17.9 bits, see alignment (E = 6.1e-07) PF00930: DPPIV_N" amino acids 247 to 350 (104 residues), 27.3 bits, see alignment E=3.8e-10

Best Hits

Swiss-Prot: 98% identical to TOLB_DICD3: Tol-Pal system protein TolB (tolB) from Dickeya dadantii (strain 3937)

KEGG orthology group: K03641, TolB protein (inferred from 98% identity to ddd:Dda3937_04502)

MetaCyc: 83% identical to Tol-Pal system periplasmic protein TolB (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"tolB protein precursor, periplasmic protein involved in the tonb-independent uptake of group A colicins" in subsystem Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4D9F1 at UniProt or InterPro

Protein Sequence (428 amino acids)

>DZA65_RS06910 Tol-Pal system protein TolB (Dickeya dianthicola ME23)
MKQALKIALGFLMLWTAVLHAEVRIEITQGVDSARPIGVVPFKSTGGAVPEDIGSIVAAD
LRNSGKFNPIDPSRMPQQPGSAAEVTPAAWTALGIDAVIVGQVQPTADGYLVSYQLVDTS
GSAGSVLAQNQFKVTKQWLRYAAHTASDEVFEKLSGIKGAFRTRIAYVVQTNGGQYPYEL
RVADYDGYNQFVVHRSPEPLMSPSWSADGSKLAYVTFESGRSALVVQTLANGDIKQIASF
PRHNGAPAFSPDGSKLAFALSKSGSLNLYVMNLGSGQISPVTDGRSNNTEPTWFPDSQTL
AYTSDQAGRPQIYKIGINGGAPQRLTWEGSQNQDAEISSDGKFLVMVSSNGGAQHIAKQD
LVTGGVQVLTDTFLDETPSIAPNGTMIIYSSKQGLGSVLQLVSTDGRFKARLPATDGQVR
FPAWSPYM