Protein Info for DZA65_RS06845 in Dickeya dianthicola ME23

Annotation: 2-oxoglutarate dehydrogenase E1 component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF16078: 2-oxogl_dehyd_N
  • amino acids 12 to 49 (38 residues), 56.3 bits, see alignment (E = 3.6e-19)
• TIGR00239: oxoglutarate dehydrogenase (succinyl-transferring), E1 component
  • amino acids 14 to 932 (919 residues), 1585.3 bits, E=0
• PF00676: E1_dh
  • amino acids 221 to 508 (288 residues), 155.6 bits, E=2.9e-49
• PF02779: Transket_pyr
  • amino acids 594 to 785 (192 residues), 174.5 bits, E=3.4e-55
• PF16870: OxoGdeHyase_C
  • amino acids 790 to 932 (143 residues), 184.1 bits, E=2.8e-58

Best Hits

Swiss-Prot: 87% identical to ODO1_ECOL6: 2-oxoglutarate dehydrogenase E1 component (sucA) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K00164, 2-oxoglutarate dehydrogenase E1 component [EC: 1.2.4.2] (inferred from 96% identity to ddc:Dd586_1187)

MetaCyc: 87% identical to subunit of E1(0) component of 2-oxoglutarate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Oxoglutarate dehydrogenase (succinyl-transferring). [EC: 1.2.1.105, 1.2.4.2]

Predicted SEED Role

"2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2)" in subsystem TCA Cycle (EC 1.2.4.2)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• 2-oxoglutarate decarboxylation to succinyl-CoA (3/3 steps found)
• TCA cycle III (animals) (8/10 steps found)
• TCA cycle VIII (Chlamydia) (5/6 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• L-lysine degradation XI (1/5 steps found)
• L-lysine degradation II (L-pipecolate pathway) (2/9 steps found)
• L-tryptophan degradation III (eukaryotic) (3/15 steps found)
• superpathway of L-lysine degradation (11/43 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Lysine degradation
• Tryptophan metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.105 or 1.2.4.2

Sequence Analysis Tools

See A0A3A4C975 at UniProt or InterPro

Protein Sequence (935 amino acids)

>DZA65_RS06845 2-oxoglutarate dehydrogenase E1 component (Dickeya dianthicola ME23)
MQNGAMKAWLDSSYLAGANQSYIEQLYEDFLTDPDSIDHSWRSIFQQLPTTGVKPDQLHS
KTRDYFRRLAKDASRYTSSVTDPDSDVKQVKVLQLINAFRFRGHQHANLDPLGLWKQEHV
SDLDLAYHNLTDDDLKESFNVGSFAIGKETMPLGELYAALKQTYCGPIGAEYMHITNTEE
KRWIQQRIESVVGQANFTAEERKRFLKELTAAEGLERYLGAKFPGAKRFSLEGGDALIPM
LKEIIRHAGKNGTREVVMGMAHRGRLNVLVNVMGKKPQDLFDEFSGKHKDHLGTGDVKYH
QGFSSDMETEGGKVHLALAFNPSHLEIVSPVVIGSVRARIDRLDNPSSSLVLPITIHGDA
AIAGQGVVQETLNMSKARGYEVGGTIRIVINNQIGFTTSNPRDARSTEYCTDIGKMVQAP
IFHVNADDPEAVAFVTHLALDFRNTFQRDVFIDLVCYRRHGHNEADEPSATQPVMYQKIK
KHPTPRKIYADRLEQQQMATLEDATELVNLYRDALDAGECVVDEWRPMDMHSFTWMSYLN
HEWDEPYPHKVGTKRLQELAHRISEIPEALEMQPRVAKIYNDRAEMAAGKKLFDWGGAET
LAYATLVDEGVPVRLSGEDAGRGTFFHRHAVVHNQKGGSTYTPLTYVHNAQGVFNVWDSV
LSEEAVLAFEYGYATAEPRTLTIWEAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLL
PHGYEGQGPEHSSARLERYLQLCAEQNMQVCIPSTPAQVYHMLRRQALRGMRRPLVVMSP
KSLLRHPLATSTLDELANGQFQPAIGEIDELDPKAVKRVVLCSGKVYYDLLEQRRKNEQK
DVAIVRIEQLYPFPHQAMQAALEPFAHVHDFVWCQEEPLNQGAWYCSQHHFREVIPFGAS
LRYAGRPASASPAVGYMSVHQKQQQALVDDALNVN