Protein Info for DZA65_RS06565 in Dickeya dianthicola ME23

Annotation: 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 156 PF02590: SPOUT_MTase" amino acids 1 to 154 (154 residues), 161.3 bits, see alignment E=8.9e-52 TIGR00246: rRNA large subunit m3Psi methyltransferase RlmH" amino acids 2 to 156 (155 residues), 267 bits, see alignment E=2.5e-84

Best Hits

Swiss-Prot: 97% identical to RLMH_PECCP: Ribosomal RNA large subunit methyltransferase H (rlmH) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K00783, hypothetical protein (inferred from 99% identity to dze:Dd1591_2963)

MetaCyc: 89% identical to 23S rRNA m3psi1915 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11592 [EC: 2.1.1.177]

Predicted SEED Role

"LSU m3Psi1915 methyltransferase RlmH" in subsystem Ribosome biogenesis bacterial

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.177

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XV22 at UniProt or InterPro

Protein Sequence (156 amino acids)

>DZA65_RS06565 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH (Dickeya dianthicola ME23)
MKLQLVAVGTKMPDWVQTGFTDYLRRFPKDMPFELVEIPAGKRGKNADIKRILEREGELM
LAAVGKGNRIVTLDIPGSRWETPQLAQQLERWKQDGRDISLLIGGPEGLAPQCKAAAEQS
WSLSPLTLPHPLVRVLVAESLYRAWSITTNHPYHRE