Protein Info for DZA65_RS05850 in Dickeya dianthicola ME23

Annotation: tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 355 TIGR00113: S-adenosylmethionine:tRNA ribosyltransferase-isomerase" amino acids 1 to 344 (344 residues), 524 bits, see alignment E=7.8e-162 PF02547: Queuosine_synth" amino acids 4 to 343 (340 residues), 438.4 bits, see alignment E=7.8e-136

Best Hits

Swiss-Prot: 89% identical to QUEA_PECCP: S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K07568, S-adenosylmethionine:tRNA ribosyltransferase-isomerase [EC: 5.-.-.-] (inferred from 96% identity to ddc:Dd586_1002)

MetaCyc: 83% identical to tRNA preQ134 S-adenosylmethionine ribosyltransferase-isomerase (Escherichia coli K-12 substr. MG1655)
RXN0-1342 [EC: 2.4.99.17]

Predicted SEED Role

"S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-)" in subsystem Queuosine-Archaeosine Biosynthesis (EC 5.-.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 5.-.-.-

Use Curated BLAST to search for 2.4.99.17 or 5.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CQY3 at UniProt or InterPro

Protein Sequence (355 amino acids)

>DZA65_RS05850 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA (Dickeya dianthicola ME23)
MRVADFSFELPESLIARYPQAKRSGCRLLSLDGPTGALTHGVFTDLLDKLQPGDLLVFNN
TRVIPARLFGRKASGGKLEVLVERMLDDKRVLAHVRASKAPKPGAELLLGEDESVKATML
ARHDALFEIQFDDTRDVLTILNDIGHMPLPPYIDRPDDAADRELYQTVYSQRPGAVAAPT
AGLHFDEPLLAALKEKGVDMAFVTLHVGAGTFQPVRVESIEDHIMHAEYAEVPQAVVDAV
LACKARGNRVIAVGTTSVRSLESAAQASTGEVIAPFFGDTRIFIFPGYHYQVVDALVTNF
HLPESTLIMLVSAFAGYRYTMTAYRQAVAEQYRFFSYGDAMFITRNPSAEQEQVG