Protein Info for DZA65_RS03365 in Dickeya dianthicola ME23

Annotation: SAM-dependent DNA methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 544 PF02384: N6_Mtase" amino acids 180 to 497 (318 residues), 211.2 bits, see alignment E=2.4e-66 PF07669: Eco57I" amino acids 301 to 410 (110 residues), 33.3 bits, see alignment E=5.2e-12

Best Hits

KEGG orthology group: K03427, type I restriction enzyme M protein [EC: 2.1.1.72] (inferred from 81% identity to spc:Sputcn32_3524)

Predicted SEED Role

"Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)" in subsystem Restriction-Modification System (EC 2.1.1.72)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XTB1 at UniProt or InterPro

Protein Sequence (544 amino acids)

>DZA65_RS03365 SAM-dependent DNA methyltransferase (Dickeya dianthicola ME23)
MVELEFRDKTKTLIDSLKSVCASYGLGNDGNEFKIITQAFLYKFLNDKFAYEAKKVDKTV
ANAERWEEALSQMSEAQLDKLQLRMNADTARLKPRHFITHLFNQQNKSDFAKTFDDTLMD
IAETNNEVFAVKTDGGAKVVLFDRLSEFIADSSKRNDFCRAIINKLMEFSFERIFTQKFD
FYATIFEYLIKDYNSNAGGKYAEYYTPHAVARIMAAILVPEAQRGTVRNVTCYDPSAGSG
TLLMNVAHAIGEDRCSIFTQDISQKSSSLLRLNLILNNLVHSIPYVIQGNTILHPYHKDG
QDLKRFDYIVSNPPFKMDFSDFRNALDTKENQPRFFAGIPNIPKSAKDKMAIYQLFLQHI
IYSLKPGGKAAVVVPTGFITAQSGIDKAIREHLVKNRMLAGVVSMPSNIFATTGTNVSIL
FIDAGNKEKVVLIDASGLGEKVKDGKNQKTVLTPEEEQRICEVFTNQWNEEDFSVVVSYD
EIAAKNYSFSAGQYFEVRIEYSDITPAQFAAKMKGFSDNLDDLFNQSHKLEAEIKQQLAG
LKYE