Protein Info for DZA65_RS03310 in Dickeya dianthicola ME23

Annotation: thymidine phosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 443 TIGR02643: thymidine phosphorylase" amino acids 2 to 438 (437 residues), 746 bits, see alignment E=1.4e-228 TIGR02644: pyrimidine-nucleoside phosphorylase" amino acids 5 to 422 (418 residues), 545.2 bits, see alignment E=9.6e-168 PF02885: Glycos_trans_3N" amino acids 5 to 67 (63 residues), 67.6 bits, see alignment E=9.6e-23 PF00591: Glycos_transf_3" amino acids 79 to 309 (231 residues), 183.4 bits, see alignment E=9.5e-58 PF07831: PYNP_C" amino acids 350 to 421 (72 residues), 73 bits, see alignment E=1.9e-24

Best Hits

Swiss-Prot: 84% identical to TYPH_PECAS: Thymidine phosphorylase (deoA) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K00758, thymidine phosphorylase [EC: 2.4.2.4] (inferred from 96% identity to ddd:Dda3937_02327)

MetaCyc: 80% identical to thymidine phosphorylase (Escherichia coli K-12 substr. MG1655)
Pyrimidine-nucleoside phosphorylase. [EC: 2.4.2.2, 2.4.2.3]; Thymidine phosphorylase. [EC: 2.4.2.2, 2.4.2.3, 2.4.2.4]

Predicted SEED Role

"Thymidine phosphorylase (EC 2.4.2.4)" in subsystem Deoxyribose and Deoxynucleoside Catabolism (EC 2.4.2.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.3

Use Curated BLAST to search for 2.4.2.2 or 2.4.2.3 or 2.4.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CVS4 at UniProt or InterPro

Protein Sequence (443 amino acids)

>DZA65_RS03310 thymidine phosphorylase (Dickeya dianthicola ME23)
MFLIQEIIRKKRDGHPLSAEEIRFFINGVRDNTVSEGQIAALAMTVFFHDMNMDERVALT
LAMRDSGTVLDWSGLNLNGPLVDKHSTGGVGDVTSLMLGPMVAACGGYVPMISGRGLGHT
GGTLDKLESIPGLDIFPEDDVFRQIIRQVGVAIIGQTSSLAPADKRFYATRDITATVDSI
ALITASILSKKLAEGLDALVMDVKVGSGAFMPTYEQSEQLARAIVGVANNAGCRTSALLT
DMNQVLASSAGNALEVREAVRFLTGEQRNPRLFEVTMALCESMLLAGNLARDAAEARAKL
QAVLDNGRAAEVFGRMVAAQHGPADFVERYDSYLSPATLSKPVFAEREGFVGAMDARALG
MVVVALGGGRRQASDIIDSSVGLSDMVSLGDWVDAQRPLAVVHARTESDWQQTAQALREA
ICLDDQPPVPCPLIYRRLDIADI