Protein Info for DZA65_RS03230 in Dickeya dianthicola ME23

Annotation: transcription termination/antitermination protein NusA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 PF08529: NusA_N" amino acids 4 to 123 (120 residues), 130.2 bits, see alignment E=9.6e-42 TIGR01953: transcription termination factor NusA" amino acids 4 to 342 (339 residues), 430.2 bits, see alignment E=5.1e-133 PF13184: KH_5" amino acids 230 to 297 (68 residues), 97.9 bits, see alignment E=5.8e-32 TIGR01954: transcription termination factor NusA, C-terminal duplication" amino acids 365 to 414 (50 residues), 71.6 bits, see alignment 4.5e-24 amino acids 441 to 489 (49 residues), 63.3 bits, see alignment 1.8e-21 PF14520: HHH_5" amino acids 367 to 411 (45 residues), 21 bits, see alignment 7.6e-08 amino acids 432 to 486 (55 residues), 46.3 bits, see alignment 1e-15

Best Hits

Swiss-Prot: 88% identical to NUSA_ECO57: Transcription termination/antitermination protein NusA (nusA) from Escherichia coli O157:H7

KEGG orthology group: K02600, N utilization substance protein A (inferred from 98% identity to dze:Dd1591_3466)

Predicted SEED Role

"Transcription termination protein NusA" in subsystem NusA-TFII Cluster or Transcription factors bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4D7L2 at UniProt or InterPro

Protein Sequence (496 amino acids)

>DZA65_RS03230 transcription termination/antitermination protein NusA (Dickeya dianthicola ME23)
MNKEILAVVEAVSNEKAVPREKIFEALETALATATKKKYEQEIDVRVCIDRKTGDFDTFR
RWLVVNEVTQPTREITLEAAQFEEPSMDLGGYIEDQIESVTFDRITTQTAKQVIVQKVRE
AERAMVVDQFREQEGEIVTGVVKKVNRDNISLDLGSNAEAVIGREDMLPRENFRPGDRIR
GVLYAVRPEARGAQLFVSRSRPEMLIELFRIEVPEIGEEVIEIKAAARDPGSRAKIAVKT
NDKRIDPVGACVGMRGARVQAVSSELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDE
DKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMTIEDLQAKHQAEAHAAIDIFT
KHLDIDEEFATALVEEGFSSLEELAYVPIHELQDIDGLDEETIEALRERAKAALTTLALA
NEENRGSGQPAEDLLNLSGLSRELAFKLAACGVCTLEDLAEQGVDDLADIEGLGEEQAGE
LIMAARNICWFGGSNE