Protein Info for DZA65_RS03020 in Dickeya dianthicola ME23

Annotation: signal transduction histidine-protein kinase/phosphatase UhpB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 504 transmembrane" amino acids 6 to 29 (24 residues), see Phobius details amino acids 37 to 54 (18 residues), see Phobius details amino acids 59 to 76 (18 residues), see Phobius details amino acids 82 to 101 (20 residues), see Phobius details amino acids 113 to 132 (20 residues), see Phobius details amino acids 138 to 163 (26 residues), see Phobius details amino acids 187 to 204 (18 residues), see Phobius details amino acids 211 to 250 (40 residues), see Phobius details amino acids 256 to 275 (20 residues), see Phobius details PF05231: MASE1" amino acids 17 to 244 (228 residues), 52.4 bits, see alignment E=6.6e-18 PF07730: HisKA_3" amino acids 306 to 371 (66 residues), 50.8 bits, see alignment E=3.1e-17 PF02518: HATPase_c" amino acids 413 to 499 (87 residues), 47 bits, see alignment E=4.7e-16

Best Hits

Swiss-Prot: 83% identical to UHPB_SALTY: Signal transduction histidine-protein kinase/phosphatase UhpB (uhpB) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K07675, two-component system, NarL family, sensor histidine kinase UhpB [EC: 2.7.13.3] (inferred from 96% identity to ddd:Dda3937_03549)

MetaCyc: 79% identical to sensor histidine kinase UhpB (Escherichia coli K-12 substr. MG1655)
Histidine kinase. [EC: 2.7.13.3]

Predicted SEED Role

"Sensor histidine protein kinase UhpB, glucose-6-phosphate specific (EC 2.7.13.3)" in subsystem Hexose Phosphate Uptake System (EC 2.7.13.3)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XT66 at UniProt or InterPro

Protein Sequence (504 amino acids)

>DZA65_RS03020 signal transduction histidine-protein kinase/phosphatase UhpB (Dickeya dianthicola ME23)
MMRPITTWLIAAFASCFIFCAAWFCLWSISLHLVTRPDLAALLFPFGLRLGLMLQSPRRF
WPVLLGSEWLLLVWLAQEVALAHLPLLLTGSVLALLPVMLASRRARQDDWHTLLLQGGAL
IAAALLQSLPWLGRGEDALTVLLLTLTGGLTLSPTCLLIWHYLSSSTWLPLGPSLVSQPV
NWRGRHLLWYLLLFVVSLWLQLGLPAELSRFTPFCLALPIIALAWHYGWQGALIATLMNA
IALIASQTWHEHPVDLLLSLLAQSLTGLLLGAGIQRLRELNLSLQNQLARNRRLAERLLA
TEEYVRRDVARELHDDIGQTITAIRTQASILQRLAPEHRGVRQSGALIEQLSLGVYDSVR
RLMGRLRPRQLDDLTLEQAVRSLMREMELEAHGIISHLAWHVDEASLGEGLRVTLFRVCQ
EGLNNIVKHANASAVTLQAWQQDGRLTLVIEDDGGGLPPGSGAEGFGLLGMRERVTALGG
TLQLSCTHGTRLTVSLPLPASKES