Protein Info for DZA65_RS02850 in Dickeya dianthicola ME23

Annotation: aspartate ammonia-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00839: aspartate ammonia-lyase
  • amino acids 6 to 474 (469 residues), 862 bits, E=6.1e-264
• PF00206: Lyase_1
  • amino acids 13 to 345 (333 residues), 347.3 bits, E=9.1e-108
• PF10415: FumaraseC_C
  • amino acids 411 to 464 (54 residues), 63.6 bits, 1.8e-21

Best Hits

Swiss-Prot: 88% identical to ASPA_SERMA: Aspartate ammonia-lyase (aspA) from Serratia marcescens

KEGG orthology group: K01744, aspartate ammonia-lyase [EC: 4.3.1.1] (inferred from 98% identity to ddd:Dda3937_02718)

MetaCyc: 87% identical to aspartate ammonia-lyase (Escherichia coli K-12 substr. MG1655)
Aspartate ammonia-lyase. [EC: 4.3.1.1]

Predicted SEED Role

"Aspartate ammonia-lyase (EC 4.3.1.1)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 4.3.1.1)

MetaCyc Pathways

• superpathway of L-aspartate and L-asparagine biosynthesis (4/4 steps found)
• L-aspartate degradation II (aerobic) (3/3 steps found)
• L-aspartate degradation III (anaerobic) (3/3 steps found)
• L-glutamate degradation II (2/2 steps found)
• L-canavanine degradation II (1/6 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.1.1

Sequence Analysis Tools

See A0A3A4CFW6 at UniProt or InterPro

Protein Sequence (479 amino acids)

>DZA65_RS02850 aspartate ammonia-lyase (Dickeya dianthicola ME23)
MSDNIRIEEDLLGTKEVPADAYYGVHTLRAIENFYISNQKISDIPEFVRGMVMVKKAAAT
ANRELQTIPRKIADIIIKACDEVLINGKCLDQFPVDVYQGGAGTSVNMNTNEVLANIGLE
LMGHKKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYNCILKLVEAVTVLGDGFEQKAKE
FENILKMGRTQLQDAVPMSLGQEFHAFNVLLKEENKNLLRTAELLLEVNLGATAIGTRLN
TPEGYQQLAVQKLAEVSKLPCVPAEDLIEATSDCGAYVMVHSTLKRLAVKLSKICNDLRL
LSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPEVVNQVCFKVIGNDICVTMAAEAGQLQ
LNVMEPVIGQAMFESTQILTNACYNLLEKCVEGITANKDVCESYVFNSIGIVTYLNPFIG
HHNGDIVGKVCAETGKSVREVVLERGLLTEAELDDIFSVQNLMHPAYKAKRYTEDQDAS