Protein Info for DZA65_RS02540 in Dickeya dianthicola ME23

Annotation: amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 389 TIGR01891: amidohydrolase" amino acids 13 to 369 (357 residues), 335.8 bits, see alignment E=1.7e-104 PF01546: Peptidase_M20" amino acids 70 to 380 (311 residues), 154.9 bits, see alignment E=2.8e-49 PF07687: M20_dimer" amino acids 185 to 275 (91 residues), 48.1 bits, see alignment E=1e-16

Best Hits

KEGG orthology group: K01451, hippurate hydrolase [EC: 3.5.1.32] (inferred from 94% identity to ddd:Dda3937_02661)

MetaCyc: 48% identical to beta-tabtoxin peptidase (Pseudomonas syringae)
3.4.11.-

Predicted SEED Role

"N-acetyl-L,L-diaminopimelate deacetylase (EC 3.5.1.47)" in subsystem Lysine Biosynthesis DAP Pathway (EC 3.5.1.47)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.32

Use Curated BLAST to search for 3.5.1.32 or 3.5.1.47

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XXJ2 at UniProt or InterPro

Protein Sequence (389 amino acids)

>DZA65_RS02540 amidohydrolase (Dickeya dianthicola ME23)
MTEIPRHIIEQAVEWRRDFHVHPEIGLREHRTAARVAELLASFGLEVHQGMAQTGVVGTL
RHGDGPAIGLRADMDALPIQELNRFSHRSRAEGCMHACGHDGHTAMLLAAARYLSDTRRF
RGTVHFVFQPAEENAGGGKMMVREGLFERFPMQAIYALHNWPGLAAGEVAVNPGSMMASQ
DSFCIILTGVGCHAAMPERGADPIVAAAQLILALQTITARRLSPLEQAVISITRIEGGEA
VNAIPGQVTLAGTLRCLTADTRERARQLIGEYVQAVPQPMGVQGELHWRSGYPVTQNQPD
AAETVREAAIAALGAARVHWNQPPSMAAEDFSYLLQACPGAYFWLGADGASPSASLHNAE
YDFNDEIIADGVRVWVALVERSLRASEAG