Protein Info for DZA65_RS01255 in Dickeya dianthicola ME23

Annotation: phosphoribosylamine--glycine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 429 PF02844: GARS_N" amino acids 1 to 102 (102 residues), 133.1 bits, see alignment E=1.1e-42 TIGR00877: phosphoribosylamine--glycine ligase" amino acids 1 to 423 (423 residues), 609.9 bits, see alignment E=1.2e-187 PF01071: GARS_A" amino acids 103 to 296 (194 residues), 275.8 bits, see alignment E=4.3e-86 PF02786: CPSase_L_D2" amino acids 115 to 249 (135 residues), 22.4 bits, see alignment E=1.6e-08 PF02843: GARS_C" amino acids 332 to 421 (90 residues), 111.2 bits, see alignment E=5e-36

Best Hits

Swiss-Prot: 86% identical to PUR2_YERPE: Phosphoribosylamine--glycine ligase (purD) from Yersinia pestis

KEGG orthology group: K01945, phosphoribosylamine--glycine ligase [EC: 6.3.4.13] (inferred from 97% identity to ddd:Dda3937_00243)

MetaCyc: 82% identical to phosphoribosylamine--glycine ligase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylamine--glycine ligase. [EC: 6.3.4.13]

Predicted SEED Role

"Phosphoribosylamine--glycine ligase (EC 6.3.4.13)" in subsystem De Novo Purine Biosynthesis (EC 6.3.4.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4C8Z9 at UniProt or InterPro

Protein Sequence (429 amino acids)

>DZA65_RS01255 phosphoribosylamine--glycine ligase (Dickeya dianthicola ME23)
MNILIIGNGGREHALAWKAAQSPLADKVYVAPGNAGTALEPALENVNIAATDIPALLDFA
RQNAIGLTIVGPEAPLVIGVVDAFRAAGLKIFGPTQAAAQLEGSKAFTKDFLARQHIPTS
EYQNFTEIEPALAYIRRKGAPIVIKADGLAAGKGVIVAMTLEEAENAATDMLAGNAFGDA
GHRIVVEDFLDGEEASFIVMVDGEHVLPMATSQDHKRVGDKDTGPNTGGMGAYSPAPVVT
DDVHQRVMDQIIWPTVRGMAAEGNVYTGFLYAGLMISPDGQPKVIEFNCRFGDPETQPIM
LRLKSDLVELCLAACDGKLDEKTSDWDPRPSLGVVLAAGGYPADYRNGDVISGLPTQDAA
DGKVFHAGTKLNGADVVTNGGRVLCVTALGHSVAEAQQRAYELAKPIRWEGSFYRSDIGY
RAIAREQQA