Protein Info for DZA65_RS00700 in Dickeya dianthicola ME23

Annotation: glutathione ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 518 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details PF00496: SBP_bac_5" amino acids 72 to 432 (361 residues), 318 bits, see alignment E=4.5e-99

Best Hits

Swiss-Prot: 42% identical to GSIB_ECO57: Glutathione-binding protein GsiB (gsiB) from Escherichia coli O157:H7

KEGG orthology group: K13889, glutathione transport system substrate-binding protein (inferred from 98% identity to ddd:Dda3937_03251)

MetaCyc: 42% identical to glutathione ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
RXN0-11 [EC: 7.4.2.10]

Predicted SEED Role

"Dipeptide-binding ABC transporter, periplasmic substrate-binding component (TC 3.A.1.5.2)" in subsystem ABC transporter dipeptide (TC 3.A.1.5.2) or Bacterial Chemotaxis (TC 3.A.1.5.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4CH87 at UniProt or InterPro

Protein Sequence (518 amino acids)

>DZA65_RS00700 glutathione ABC transporter substrate-binding protein (Dickeya dianthicola ME23)
MKPLLSAVTSRRSAVALGLSLCLAAAAQAQDLRISMYADITGLDPHDTSDTLSYSIQSGI
FERLFQFDNQMKLVPRLATGYTGNADATEFTITLREGITFQDGTPFNAEAVKANLDRLAD
QTKGLKRNSLFNMIKSVTVLSPTQVKIELNKSFGAFVNTLAHPSAVMHSPAALKQYPDET
QLRVHPVGTGPFTFSEWQQGKDVKLVKYDNYWQKGWPKVDSITFYPSPEDATRVAALKSG
QSDAIYPLPSDLVKTVEDDAKLAIQRDPSIYLYYMAINTQHAPLNDVRVRQALNYAINRT
IWLKVGFAGMGLPAASTMAPRVQFFASQSEPNYSYSPAKAKELLKEAGYENGLELKLWTT
NTTAAVRSAQFFKQQLEQVGIKATVTPMDSGARNEKLWGVKDPKQAEFDLYYGGWSPSTG
DADWALRPLFATESWVPKAYNVSYYSSPDVDKAIMAGLATPDNDKRAAAYADAQKLIWKD
APVVFLGVPDNLVGKVKNLSGVYMLADGSLIFDQAEFK