Protein Info for DZA65_RS00375 in Dickeya dianthicola ME23

Annotation: HAD family hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 230 PF00702: Hydrolase" amino acids 4 to 181 (178 residues), 101.9 bits, see alignment E=1.2e-32 PF13419: HAD_2" amino acids 5 to 187 (183 residues), 129.4 bits, see alignment E=3.5e-41 PF12710: HAD" amino acids 5 to 178 (174 residues), 48.9 bits, see alignment E=2.3e-16 TIGR01509: HAD hydrolase, family IA, variant 3" amino acids 89 to 187 (99 residues), 35.4 bits, see alignment E=1.1e-12 TIGR01549: HAD hydrolase, family IA, variant 1" amino acids 99 to 181 (83 residues), 32.2 bits, see alignment E=1.3e-11 PF13242: Hydrolase_like" amino acids 144 to 211 (68 residues), 52.5 bits, see alignment E=7.8e-18

Best Hits

KEGG orthology group: K01091, phosphoglycolate phosphatase [EC: 3.1.3.18] (inferred from 91% identity to ddd:Dda3937_00973)

Predicted SEED Role

"Phosphoglycolate phosphatase (EC 3.1.3.18)" in subsystem 2-phosphoglycolate salvage or Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 3.1.3.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.18

Use Curated BLAST to search for 3.1.3.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XV30 at UniProt or InterPro

Protein Sequence (230 amino acids)

>DZA65_RS00375 HAD family hydrolase (Dickeya dianthicola ME23)
MKPLVIFDLDGTLVDTPSGIVSAFVTTLRDLGMPFEDRRAIRATIGMPLEKAFGQILALP
LEDESVAAAIRQYQAVFREQVLPQAPGLVFPGVEEGLTLLRRQGYALAVATSKVFVSAKA
LLEAAGLWSYFDLVLGADMVTHPKPHPEMGLLAMSRLGADAATTAMVGDTTHDLLMAKQA
GMVAIGVTWGIHHTDQLKAAEPQVIVDTFGEVVGAAHALLKRSSSPASYC