Protein Info for DZA65_RS00265 in Dickeya dianthicola ME23

Annotation: PTS mannitol transporter subunit IICBA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 635 transmembrane" amino acids 21 to 43 (23 residues), see Phobius details amino acids 52 to 73 (22 residues), see Phobius details amino acids 80 to 105 (26 residues), see Phobius details amino acids 134 to 155 (22 residues), see Phobius details amino acids 174 to 182 (9 residues), see Phobius details amino acids 188 to 190 (3 residues), see Phobius details amino acids 214 to 235 (22 residues), see Phobius details amino acids 246 to 264 (19 residues), see Phobius details amino acids 270 to 293 (24 residues), see Phobius details amino acids 312 to 335 (24 residues), see Phobius details TIGR00851: PTS system, mannitol-specific IIC component" amino acids 9 to 347 (339 residues), 602 bits, see alignment E=1.4e-185 PF02378: PTS_EIIC" amino acids 18 to 278 (261 residues), 82.3 bits, see alignment E=5.5e-27 PF02302: PTS_IIB" amino acids 377 to 454 (78 residues), 44.3 bits, see alignment E=3.5e-15 PF00359: PTS_EIIA_2" amino acids 492 to 630 (139 residues), 119.8 bits, see alignment E=1.4e-38

Best Hits

KEGG orthology group: K02798, PTS system, mannitol-specific IIA component [EC: 2.7.1.69] K02799, PTS system, mannitol-specific IIB component [EC: 2.7.1.69] K02800, PTS system, mannitol-specific IIC component (inferred from 97% identity to ddd:Dda3937_00995)

Predicted SEED Role

"PTS system, mannitol-specific IIC component (EC 2.7.1.69) / PTS system, mannitol-specific IIB component (EC 2.7.1.69) / PTS system, mannitol-specific IIA component" in subsystem Mannitol Utilization (EC 2.7.1.69)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A385XV12 at UniProt or InterPro

Protein Sequence (635 amino acids)

>DZA65_RS00265 PTS mannitol transporter subunit IICBA (Dickeya dianthicola ME23)
MLSPDTKVKVQNFGRFLSNMVMPNIGAFIAWGIITALFIPTGWFPNATLAKLVGPMITYL
LPLLIGFTGGRLVGGERGGVVGAITTMGVVVGSDIPMFLGAMIAGPLGGWAIKHFDAAVD
GKIKSGFEMLVNNFSAGIIGMLLAILSFLAIGPLVEALSRILAAGVHLMVQNNLLPLASI
FVEPAKILFLNNAINHGIFSPLGIQQATEAGKSIFFLIEANPGPGMGVLMAYMLFGRGNA
KQSAPGAAIIHFLGGIHEIYFPYVLMNPRLIIAVILGGMTGVFTLGLLNGGLVSPASPGS
ILAVLAMTPKGAYFANIAAIAAAFAVSFVVSAILLKSSKVKDDDELELATRRMQEMKSAS
KGQAVSGVSGDLGTVRKIIVACDAGMGSSAMGAGVLRKKVQDAGLRNISVTNSAINNLPD
DVDLVITHRDLTERAMRHAPQAQHISLTNFLDSHLYNDLVARLQAAQPGDAKPAAAAAVA
EPAAAPQALFQLTEKNVFLNLHAADKEQAIRFAGEQLVKGGYVEPAYVDAMLEREKLTST
YLGESIAVPHGTIEAKDRVLKTGVVFCQYPQGVRFGDDEDDVARLVIGIAARNNEHVQVI
TNLTSALDDESIIERLAQTENVQDVLDLLSGKSPA