Protein Info for DZA65_RS00150 in Dickeya dianthicola ME23

Annotation: glucokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 322 transmembrane" amino acids 248 to 270 (23 residues), see Phobius details PF02685: Glucokinase" amino acids 6 to 316 (311 residues), 423.7 bits, see alignment E=2e-131 TIGR00749: glucokinase" amino acids 6 to 311 (306 residues), 433.6 bits, see alignment E=2.2e-134

Best Hits

Swiss-Prot: 73% identical to GLK_YERE8: Glucokinase (glk) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

KEGG orthology group: K00845, glucokinase [EC: 2.7.1.2] (inferred from 97% identity to ddd:Dda3937_01026)

MetaCyc: 71% identical to glucokinase (Escherichia coli K-12 substr. MG1655)
Glucosamine kinase. [EC: 2.7.1.8]; Hexokinase. [EC: 2.7.1.8, 2.7.1.1, 2.7.1.2]

Predicted SEED Role

"Glucokinase (EC 2.7.1.2)" in subsystem Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis (EC 2.7.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.8

Use Curated BLAST to search for 2.7.1.1 or 2.7.1.2 or 2.7.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A3A4D6D6 at UniProt or InterPro

Protein Sequence (322 amino acids)

>DZA65_RS00150 glucokinase (Dickeya dianthicola ME23)
MHQYALVGDVGGTNARLALCELANGQLSHSKQYAVQQHDSLEDAMRLFLAEHASLTIKEA
CIAIACPVTDDWVEMTNHHWAFSIAAMRQNLGFERLEVINDFTAVSMAIPVLTPEDVIQL
GGADAVAGKPVAVYGAGTGLGVAHLLPVAGTWLSLPGEGGHVDFASNSEEEDILLQVLRQ
ELGHVSAERVLSGPGLVNIYRAIVKADNRVPEALTPQVVSERALAHSDVDCLRALSLFCV
LMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFRNSGFRSAFEDKGRFRDYLADIPVFMI
THPQPGLLGAGAYLRQTLGQTL