Protein Info for DDA3937_RS21535 in Dickeya dadantii 3937

Annotation: GTP cyclohydrolase II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 57% identical to RIBA_ALISL: GTP cyclohydrolase-2 (ribA) from Aliivibrio salmonicida (strain LFI1238)

KEGG orthology group: K01497, GTP cyclohydrolase II [EC: 3.5.4.25] (inferred from 100% identity to ddd:Dda3937_00140)

MetaCyc: 57% identical to GTP cyclohydrolase II (Burkholderia glumae)
GTP cyclohydrolase II. [EC: 3.5.4.25]

Predicted SEED Role

"3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25)" in subsystem Molybdenum cofactor biosynthesis or Riboflavin, FMN and FAD metabolism (EC 3.5.4.25, EC 4.1.99.12)

MetaCyc Pathways

flavin biosynthesis I (bacteria and plants) (9/9 steps found)
flavin biosynthesis III (fungi) (8/9 steps found)
6-hydroxymethyl-dihydropterin diphosphate biosynthesis III (Chlamydia) (3/5 steps found)
toxoflavin biosynthesis (4/7 steps found)
flavin biosynthesis II (archaea) (6/10 steps found)

KEGG Metabolic Maps

Riboflavin metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.25, 4.1.99.12

Use Curated BLAST to search for 3.5.4.25 or 4.1.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SDR6 at UniProt or InterPro

Protein Sequence (208 amino acids)

>DDA3937_RS21535 GTP cyclohydrolase II (Dickeya dadantii 3937)
MSDSHQAAHEVLVASKVQIPITIGDWSDNGAQFYGFENLVDGKEHIAIVFGDVENKSHVL
VRMHSECLTGDVFGSLRCDCGPQLHESLKSLSASGGVLLYLRQEGRGIGLINKLKAYELQ
RKGMNTYEANNELGFPDDLRNYESAAQMLKALNVTSISLITNNPDKAKQLRAYGITVVEM
KNTGCHSNENNLDYLKAKKEHTHHTLSI