Protein Info for DDA3937_RS20035 in Dickeya dadantii 3937

Annotation: lipid III flippase WzxE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 416 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details transmembrane" amino acids 23 to 24 (2 residues), see Phobius details amino acids 46 to 67 (22 residues), see Phobius details amino acids 79 to 102 (24 residues), see Phobius details amino acids 121 to 139 (19 residues), see Phobius details amino acids 149 to 169 (21 residues), see Phobius details amino acids 175 to 195 (21 residues), see Phobius details amino acids 217 to 238 (22 residues), see Phobius details amino acids 263 to 281 (19 residues), see Phobius details amino acids 293 to 314 (22 residues), see Phobius details amino acids 334 to 355 (22 residues), see Phobius details amino acids 362 to 382 (21 residues), see Phobius details amino acids 394 to 413 (20 residues), see Phobius details PF01943: Polysacc_synt" amino acids 4 to 284 (281 residues), 38.4 bits, see alignment E=1e-13 PF13440: Polysacc_synt_3" amino acids 80 to 330 (251 residues), 38.6 bits, see alignment E=7.9e-14

Best Hits

Swiss-Prot: 60% identical to WZXE_ECOLI: Lipid III flippase (wzxE) from Escherichia coli (strain K12)

KEGG orthology group: K03328, polysaccharide transporter, PST family (inferred from 100% identity to ddd:Dda3937_00274)

MetaCyc: 60% identical to lipid IIIECA flippase (Escherichia coli K-12 substr. MG1655)
7.5.99.a [EC: 7.5.99.a]

Predicted SEED Role

"WzxE protein"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.5.99.a

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SKQ1 at UniProt or InterPro

Protein Sequence (416 amino acids)

>DDA3937_RS20035 lipid III flippase WzxE (Dickeya dadantii 3937)
MSLARASLWTAASTLVKIGAGLAVIKLLAVTFGPEGVGLAGNYRQLITVLGVMAGAGIAN
GVTRAVAAAPPDANRPGPLLGTAVSLSMGCSLLLTLALWLLAAPLSRLLFGDDAYQPAIR
ALAWLQLGIAGASLLLAILKGYQDARGNALAVMAGSLLGAVAYGVSVWLGAYTGALVGLA
LMPALTCVPTLILLLRRTPLSLQALTPDWSWPLAGQLTRFSLMTLITAVTLPVGYVMMRN
LLATHYSWQEVGVWQGVTTISDAWLQFITALFTVYLLPALARLQDKRAVRHEILSALRFV
LPVATAVGVAIWLWRDVAIRLLFSSAFSAMRDLFAWQLAGDVLKVGAYVFGYLVVARASL
RFYLLAELGQFLLLTGFSRWLIPLHGALGASQAYLATYAVYFLLCCGVFTLYCRRT