Protein Info for DDA3937_RS19450 in Dickeya dadantii 3937
Annotation: aspartate aminotransferase family protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 82% identical to ARGD_YERPE: Acetylornithine/succinyldiaminopimelate aminotransferase (argD) from Yersinia pestis
KEGG orthology group: K00821, acetylornithine/N-succinyldiaminopimelate aminotransferase [EC: 2.6.1.11 2.6.1.17] (inferred from 100% identity to ddd:Dda3937_00547)MetaCyc: 75% identical to N-acetylornithine aminotransferase / N-succinyldiaminopimelate aminotransferase (Escherichia coli K-12 substr. MG1655)
Succinyldiaminopimelate transaminase. [EC: 2.6.1.17]; Acetylornithine transaminase. [EC: 2.6.1.17, 2.6.1.11]
Predicted SEED Role
"Acetylornithine aminotransferase (EC 2.6.1.11) / N-succinyl-L,L-diaminopimelate aminotransferase (EC 2.6.1.17)" in subsystem Arginine Biosynthesis extended or Lysine Biosynthesis DAP Pathway (EC 2.6.1.11, EC 2.6.1.17)
MetaCyc Pathways
- aspartate superpathway (24/25 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (18/18 steps found)
- L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
- L-lysine biosynthesis I (9/9 steps found)
- L-arginine biosynthesis II (acetyl cycle) (9/10 steps found)
- superpathway of arginine and polyamine biosynthesis (14/17 steps found)
- L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
- L-ornithine biosynthesis I (5/5 steps found)
KEGG Metabolic Maps
- Arginine and proline metabolism
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Lysine biosynthesis
- Urea cycle and metabolism of amino groups
Isozymes
Compare fitness of predicted isozymes for: 2.6.1.11
Use Curated BLAST to search for 2.6.1.11 or 2.6.1.17
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See E0SJP5 at UniProt or InterPro
Protein Sequence (407 amino acids)
>DDA3937_RS19450 aspartate aminotransferase family protein (Dickeya dadantii 3937) MATEKAAVTRDTYDKVILPVYAPAQFVPVKGKGSRVWDQQGKEYIDFSGGIAVTALGHCH PALVAALKEQGETLWHTSNVFTNEPALRLATKLINATFADRVFFANSGAEANEAAFKLAR HYAITRHSPYKTKIISFYQSFHGRTLFTVSVGGQPKYADGFGPKPADIVHVPFNDLDALK AVMDDHTCAVVLEPIQGEGGITPATPAFLSGVRELCDQYQALLVFDEVQCGMGRSGKLFS YMHYGITPDILTTAKALGGGFPISAMLTTEEIASVMAVGTHGTTYGGNPLACAVAEAALD VINTPEVLSGVMDRHARFVSALERINAQYDIFDEIRGMGLLLGAQLKPAWHGRARDFLAA SADLGLMILVAGPDVIRFVPSLVITPEEIDQGMALFGKAVEQVVKGA