Protein Info for DDA3937_RS18855 in Dickeya dadantii 3937

Annotation: Fe-S cluster assembly protein NifU

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 275 TIGR02000: Fe-S cluster assembly protein NifU" amino acids 1 to 275 (275 residues), 460.7 bits, see alignment E=1.1e-142 PF01592: NifU_N" amino acids 4 to 125 (122 residues), 158.5 bits, see alignment E=1.3e-50 PF04324: Fer2_BFD" amino acids 135 to 184 (50 residues), 63.3 bits, see alignment 3.2e-21 PF01106: NifU" amino acids 209 to 272 (64 residues), 64.5 bits, see alignment E=1.2e-21

Best Hits

Swiss-Prot: 81% identical to NIFU_KLEPN: Nitrogen fixation protein NifU (nifU) from Klebsiella pneumoniae

KEGG orthology group: K13819, NifU-like protein (inferred from 100% identity to ddd:Dda3937_02171)

Predicted SEED Role

"Iron-sulfur cluster assembly scaffold protein NifU" in subsystem Nitrogen fixation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SI03 at UniProt or InterPro

Protein Sequence (275 amino acids)

>DDA3937_RS18855 Fe-S cluster assembly protein NifU (Dickeya dadantii 3937)
MWNYSEKVKDHFFNPRNARVVAEANAVGDVGSLSCGDALRLMLRVDPDSETILDAGFQTF
GCGSAIASSSALTELIIGRTLREAEQVTNQQIADYLDGLPPEKMHCSVMGQEALRVAIAN
YRGETLEDDHEEGALICKCFAVDEGQIRRAVVANGLTTLQEVINYTKAGGGCSACHEKIE
LALAQILSEHEATPAAAAIVVKDARWQQVAEAVAELRPHIQADGGDMSLVNVSERQVTVS
LSGSCNGCMMTDMTLAWLQQKLVERTGQYMDVVAA