Protein Info for DDA3937_RS18635 in Dickeya dadantii 3937

Annotation: bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 513 TIGR00197: YjeF family N-terminal domain" amino acids 29 to 223 (195 residues), 178.4 bits, see alignment E=1.5e-56 PF03853: YjeF_N" amino acids 44 to 203 (160 residues), 144.7 bits, see alignment E=2.8e-46 TIGR00196: YjeF family C-terminal domain" amino acids 237 to 503 (267 residues), 279.3 bits, see alignment E=3.3e-87 PF01256: Carb_kinase" amino acids 261 to 499 (239 residues), 248.6 bits, see alignment E=6.2e-78

Best Hits

Swiss-Prot: 66% identical to NNR_ECOLI: Bifunctional NAD(P)H-hydrate repair enzyme Nnr (nnr) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to ddd:Dda3937_00439)

MetaCyc: 66% identical to NAD(P)HX epimerase / NAD(P)HX dehydratase (Escherichia coli K-12 substr. MG1655)
RXN-12752 [EC: 5.1.99.6]; 5.1.99.6 [EC: 5.1.99.6]; RXN-13141 [EC: 5.1.99.6, 4.2.1.136]; 4.2.1.136 [EC: 5.1.99.6, 4.2.1.136]

Predicted SEED Role

"NAD(P)HX epimerase / NAD(P)HX dehydratase"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.136 or 5.1.99.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SH76 at UniProt or InterPro

Protein Sequence (513 amino acids)

>DDA3937_RS18635 bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase (Dickeya dadantii 3937)
MHQDHCVYQPGGSLPDLPQTVFLPDWLRREEPHAADYVGVSLNTLMQRAGDAAFRIAQQQ
YPTARHWLILCGHGNNGGDGYVVAALARAAGVAVTVVAGSGDRSLPAEAHLARQAWCDAG
GCEQHVDALWPDDIDLIIDGLLGTGLTSAPRAPYDALIAQANRHPAPIVALDIPSGLNAQ
TGACAGAAIHAAHTVTFITLKPGLLTGQARDVVGQLHHHDLGLGDWLAQQSAPIARLTAA
SLTQWLKPRRPGAHKGEHGRLLIIGGDEGTGGAIFMAGEAALRSGAGLVRVLTHKSYLAA
LLVARPELMVQELTTDSVKAGVEWADVVVIGPGLGQREWGKNALRIAENCNKSMLWDADA
LNLLAISPHKRQNRVLTPHPGEAARLLNCRIADIESDRLLAAQRLVKRYGGVVLLKGAGT
VLAGEQGELAIVDVGNPGMATGGMGDVLAGMIGAMLAQGLSLYDAACAGCVAHGAAADWL
ARRQGTRGMLATDLMAVLPQYVNPERISLENTE