Protein Info for DDA3937_RS17915 in Dickeya dadantii 3937

Annotation: dihydrolipoyl dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01350: dihydrolipoyl dehydrogenase
  • amino acids 8 to 460 (453 residues), 497.2 bits, E=2.2e-153
• PF01134: GIDA
  • amino acids 8 to 70 (63 residues), 31.4 bits, E=4.9e-11
• PF07992: Pyr_redox_2
  • amino acids 8 to 328 (321 residues), 225.2 bits, E=5.7e-70
• PF12831: FAD_oxidored
  • amino acids 9 to 45 (37 residues), 35.2 bits, 4.2e-12
• PF00890: FAD_binding_2
  • amino acids 9 to 43 (35 residues), 22.6 bits, 2.6e-08
• PF00070: Pyr_redox
  • amino acids 177 to 250 (74 residues), 61.3 bits, E=4.7e-20
• PF02852: Pyr_redox_dim
  • amino acids 347 to 455 (109 residues), 126.3 bits, E=2.9e-40

Best Hits

Swiss-Prot: 94% identical to DLDH_ECO57: Dihydrolipoyl dehydrogenase (lpdA) from Escherichia coli O157:H7

KEGG orthology group: K00382, dihydrolipoamide dehydrogenase [EC: 1.8.1.4] (inferred from 100% identity to ddd:Dda3937_03096)

MetaCyc: 94% identical to lipoamide dehydrogenase (Escherichia coli K-12 substr. MG1655)
1.97.1.-; Dihydrolipoyl dehydrogenase. [EC: 1.4.1.27, 1.8.1.4]; RXN-7716 [EC: 1.4.1.27, 1.8.1.4, 1.2.1.105]; RXN0-1132 [EC: 1.4.1.27, 1.8.1.4, 1.2.1.105, 1.2.1.104]; 1.8.1.4 [EC: 1.4.1.27, 1.8.1.4, 1.2.1.105, 1.2.1.104]; 1.4.1.27 [EC: 1.4.1.27, 1.8.1.4, 1.2.1.105, 1.2.1.104]; 1.2.1.105 [EC: 1.4.1.27, 1.8.1.4, 1.2.1.105, 1.2.1.104]; 1.2.1.104 [EC: 1.4.1.27, 1.8.1.4, 1.2.1.105, 1.2.1.104]

Predicted SEED Role

No annotation

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• folate transformations III (E. coli) (9/9 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• folate transformations II (plants) (10/11 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (4/4 steps found)
• 2-oxoglutarate decarboxylation to succinyl-CoA (3/3 steps found)
• glycine biosynthesis II (3/3 steps found)
• glycine cleavage (3/3 steps found)
• glycine degradation (3/3 steps found)
• pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
• pyruvate fermentation to acetate II (3/3 steps found)
• TCA cycle III (animals) (8/10 steps found)
• TCA cycle VIII (Chlamydia) (5/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
• pyruvate fermentation to acetate V (2/3 steps found)
• photorespiration II (7/10 steps found)
• folate transformations I (9/13 steps found)
• photorespiration I (6/9 steps found)
• photorespiration III (6/9 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)
• 2-oxoisovalerate decarboxylation to isobutanoyl-CoA (1/3 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (5/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (10/17 steps found)
• L-lysine degradation XI (1/5 steps found)
• L-lysine degradation II (L-pipecolate pathway) (2/9 steps found)
• L-tryptophan degradation III (eukaryotic) (3/15 steps found)
• superpathway of L-lysine degradation (12/43 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glycine, serine and threonine metabolism
• Glycolysis / Gluconeogenesis
• Pyruvate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.104 or 1.2.1.105 or 1.4.1.27 or 1.8.1.4

Sequence Analysis Tools

See E0SG50 at UniProt or InterPro

Protein Sequence (474 amino acids)

>DDA3937_RS17915 dihydrolipoyl dehydrogenase (Dickeya dadantii 3937)
MSTEIKAQVVVLGAGPAGYSAAFRCADLGLDTVLVERYSTLGGVCLNVGCIPSKALLHVA
KVIEEAKALAEHGIVFGEPQTDIDKIRTWKEKVINQMSGGLAGMAKGRKVKVVNGFGKFT
GPNTLVVEGESGSTTVNFDNAIIAAGSRPIQLPFIPHEDPRVWDSTDALELKSVPGRLLV
MGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDIVKVFTKRISKKFNLMLETKVTA
VEAKEDGIYVSMEGKKAPAEPQRYDAVLVAIGRVPNGKLLDAGQAGVEVDDRGFIRVDKQ
MRTNVPHIYAIGDIVGQPMLAHKGVHEGHVAAEVIAGKKHYFDPKVIPSIAYTEPEVAWV
GLTEKEAKEKGISYETAVFPWAASGRAVASDCSDGMTKLIFDKETHRVIGGAIVGTNGGE
LLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNPKAKKK