Protein Info for DDA3937_RS11940 in Dickeya dadantii 3937

Annotation: NarK family nitrate/nitrite MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 463 transmembrane" amino acids 39 to 62 (24 residues), see Phobius details amino acids 75 to 93 (19 residues), see Phobius details amino acids 105 to 125 (21 residues), see Phobius details amino acids 131 to 154 (24 residues), see Phobius details amino acids 173 to 196 (24 residues), see Phobius details amino acids 216 to 234 (19 residues), see Phobius details amino acids 255 to 278 (24 residues), see Phobius details amino acids 289 to 308 (20 residues), see Phobius details amino acids 319 to 339 (21 residues), see Phobius details amino acids 348 to 374 (27 residues), see Phobius details amino acids 404 to 425 (22 residues), see Phobius details amino acids 437 to 457 (21 residues), see Phobius details TIGR00886: nitrite transporter" amino acids 37 to 418 (382 residues), 357.7 bits, see alignment E=3.6e-111 PF07690: MFS_1" amino acids 46 to 377 (332 residues), 60.9 bits, see alignment E=5.3e-21

Best Hits

Swiss-Prot: 74% identical to NARK_ECOLI: Nitrate/nitrite transporter NarK (narK) from Escherichia coli (strain K12)

KEGG orthology group: K02575, MFS transporter, NNP family, nitrate/nitrite transporter (inferred from 100% identity to ddd:Dda3937_03788)

MetaCyc: 74% identical to nitrate:nitrite antiporter NarK (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-239

Predicted SEED Role

"Nitrate/nitrite transporter" in subsystem Nitrate and nitrite ammonification

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E0SEC0 at UniProt or InterPro

Protein Sequence (463 amino acids)

>DDA3937_RS11940 NarK family nitrate/nitrite MFS transporter (Dickeya dadantii 3937)
MTQSTITPGDSRQTVIRDWRPEDVQFWQKQGRGIAQRNLWISIPCLLLAFCVWMLFSTVA
VNLNKVGFHFTTDQLFMLTALPSVSGALLRVPYSFIIPLAGGRRWTTFSTLILVIPCVWL
GFVVQDPQTPYSVFIVISLLCGFAGANFASSMANISFFFPKAQQGSALGLNGGFGNLGVS
VMQLLVPVVIFLPVLSFTGNGVAQPDGGQVWLQNAPWVWVPLLLIASAAAWFGMNDLSTA
KASLRQQLPVLKRAHLWILSVLYLSAFGSFIGFSAGFAMLSKTQFPDVIILHYAFCGPLL
GALARPVGGMLSDRFGGVRVTLINFVFMAILSVLLFTALPGDNQPGSFLLFFGIFMLLFL
TAGLGSGSTFQMIAVIFRGLTVERVTAAGGGEDEAQRSAVTDTAAALGFISAIGAIGGFF
IPKAFGTSLAMTGSPAGAMKIFVVFYIACVVITWLCYGRKTRS